PMID- 23159147
OWN - NLM
STAT- MEDLINE
DCOM- 20131025
LR  - 20181202
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 152
IP  - 1-2
DP  - 2013 Mar 15
TI  - Optimization of a small intestinal segment perfusion model for heat-stable 
      enterotoxin A induced secretion in pigs.
PG  - 82-6
LID - S0165-2427(12)00344-3 [pii]
LID - 10.1016/j.vetimm.2012.09.014 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) are a major cause of infectious diarrhea 
      both in human and pigs. After ingestion of contaminated food or water, ETEC 
      bacteria colonize the small intestine where they produce heat-labile (LT) and/or 
      heat-stable (ST) enterotoxins, which induce watery diarrhea. We investigated the 
      possibility of eliciting STa-induced secretion in jejunal segments of 
      anesthetized pigs using a small-intestinal segment perfusion (SISP) model. Five 
      consecutive mid-jejunal segments of anaesthetized piglets were perfused for 6h 
      with different concentrations of STa in a physiologic salt solution. Changes in 
      intestinal net fluid absorption were measured. From the results we could conclude 
      that the STa response was dose-dependent and that continuous perfusion with 50 nM 
      of STa or more was required to reduce net absorption. This concentration was 
      sufficient to reduce net absorption compared to control segments in 12 out of 14 
      piglets. STa-induced responses however showed relative high variation between 
      different jejunal segments of one pig, similar to the inter-segment variation 
      seen in control animals where segments were perfused with physiologic salt 
      solution. These results indicate that more optimization is required before this 
      model could be used to test compounds that could interfere with the STa-induced 
      fluid secretion.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Loos, Michaela
AU  - Loos M
AD  - Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Ghent 
      University, Salisburylaan 133, B-9820 Merelbeke, Belgium. michaela.loos@ugent.be
FAU - Hellemans, Ann
AU  - Hellemans A
FAU - Cox, Eric
AU  - Cox E
LA  - eng
PT  - Journal Article
DEP - 20120926
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/immunology
MH  - Disease Models, Animal
MH  - Enterotoxigenic Escherichia coli/immunology/*physiology
MH  - Enterotoxins/immunology/*metabolism
MH  - Escherichia coli Infections/immunology/*microbiology/*veterinary
MH  - Escherichia coli Proteins/immunology/*metabolism
MH  - Female
MH  - In Vitro Techniques
MH  - Intestinal Absorption
MH  - Intestinal Diseases/immunology/*microbiology/*veterinary
MH  - Jejunum/*microbiology
MH  - Swine
MH  - Swine Diseases/immunology/microbiology
EDAT- 2012/11/20 06:00
MHDA- 2013/10/26 06:00
CRDT- 2012/11/20 06:00
PHST- 2012/11/20 06:00 [entrez]
PHST- 2012/11/20 06:00 [pubmed]
PHST- 2013/10/26 06:00 [medline]
AID - S0165-2427(12)00344-3 [pii]
AID - 10.1016/j.vetimm.2012.09.014 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2013 Mar 15;152(1-2):82-6. doi: 
      10.1016/j.vetimm.2012.09.014. Epub 2012 Sep 26.