PMID- 23161705
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20211021
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 2
DP  - 2013 Feb
TI  - N-methyl-D-aspartate receptor-mediated axonal injury in adult rat corpus 
      callosum.
PG  - 240-8
LID - 10.1002/jnr.23150 [doi]
AB  - Damage to white matter such as corpus callosum (CC) is a pathological 
      characteristic in many brain disorders. Glutamate (Glut) excitotoxicity through 
      AMPA receptors on oligodendrocyte (OL) was previously considered as a mechanism 
      for white matter damage. Recent studies have shown that N-methyl-D-aspartate 
      receptors (NMDARs) are expressed on myelin sheath of neonatal rat OL processes 
      and that activation of these receptors mediated demyelization. Whether NMDARs are 
      expressed in the adult CC and are involved in excitotoxic axonal injury remains 
      to be determined. In this study, we demonstrate the presence of NMDARs in the 
      adult rat CC and their distributions in myelinated nerve fibers and OL somata by 
      means of immunocytochemical staining and Western blot. Incubation of the CC 
      slices with Glut or NMDA induced axonal injury as revealed by analyzing amplitude 
      of CC fiber compound action potentials (CAPs) and input-output response. Both 
      Glut and NMDA decreased the CAP amplitude and input-output responses, suggesting 
      an involvement of NMDARs in Glut- and NMDA-induced axonal injury. The involvement 
      of NMDAR in Glut-induced axonal injury was further assayed by detection of 
      beta-amyloid precursor protein (beta-APP) in the CC axonal fibers. Treatment of the CC 
      slices with Glut resulted in beta-APP accumulation in the CC fibers as detected by 
      Western blot, reflecting an impairment of axonal transport function. This 
      injurious effect of Glut on CC axonal transport was significantly blocked by 
      MK801. Taken together, these results show that NMDARs are expressed in the adult 
      CC and are involved in excitotoxic activity in adult CC slices in vitro.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Zhang, Jingdong
AU  - Zhang J
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, Nebraska 68198-5880, USA.
FAU - Liu, Jianuo
AU  - Liu J
FAU - Fox, Howard S
AU  - Fox HS
FAU - Xiong, Huangui
AU  - Xiong H
LA  - eng
GR  - P30 MH062261/MH/NIMH NIH HHS/United States
GR  - R01 NS063878/NS/NINDS NIH HHS/United States
GR  - R01 NS077873/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121114
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Excitatory Amino Acids)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (NMDA receptor A1)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Amyloid beta-Protein Precursor/metabolism
MH  - Animals
MH  - Axons/*pathology
MH  - Corpus Callosum/*metabolism
MH  - Disease Models, Animal
MH  - Dizocilpine Maleate/pharmacology
MH  - Excitatory Amino Acids/toxicity
MH  - Female
MH  - Glutamic Acid/toxicity
MH  - In Vitro Techniques
MH  - Male
MH  - Myelin Basic Protein/metabolism
MH  - Nerve Fibers, Myelinated/pathology/physiology
MH  - Neurons/metabolism
MH  - Neuroprotective Agents/pharmacology
MH  - Neurotoxicity Syndromes/etiology/*pathology
MH  - Oligodendroglia/metabolism
MH  - Patch-Clamp Techniques
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
PMC - PMC3546500
MID - NIHMS432447
EDAT- 2012/11/20 06:00
MHDA- 2013/06/01 06:00
PMCR- 2014/02/01
CRDT- 2012/11/20 06:00
PHST- 2012/05/05 00:00 [received]
PHST- 2012/08/31 00:00 [revised]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/11/20 06:00 [entrez]
PHST- 2012/11/20 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - 10.1002/jnr.23150 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Feb;91(2):240-8. doi: 10.1002/jnr.23150. Epub 2012 Nov 14.