PMID- 23161805
OWN - NLM
STAT- MEDLINE
DCOM- 20130606
LR  - 20211021
IS  - 1757-4684 (Electronic)
IS  - 1757-4676 (Print)
IS  - 1757-4676 (Linking)
VI  - 5
IP  - 1
DP  - 2013 Jan
TI  - Strategy for the creation of clinical grade hESC line banks that HLA-match a 
      target population.
PG  - 10-7
LID - 10.1002/emmm.201201973 [doi]
AB  - Here, we describe a pre-derivation embryo haplotyping strategy that we developed 
      in order to maximize the efficiency and minimize the costs of establishing banks 
      of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes 
      match a significant proportion of the population. Using whole genome 
      amplification followed by medium resolution HLA typing using PCR amplification 
      with sequence-specific primers (PCR-SSP), we have typed the parents, embryos and 
      hESC lines from three families as well as our eight clinical grade hESC lines and 
      shown that this technical approach is rapid, reliable and accurate. By employing 
      this pre-derivation strategy where, based on HLA match, embryos are selected for 
      a GMP route on day 3-4 of development, we would have drastically reduced our cGMP 
      laboratory running costs.
CI  - Copyright (c) 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of 
      EMBO.
FAU - Jacquet, Laureen
AU  - Jacquet L
AD  - Embryonic Stem Cell Laboratories, Guy's Assisted Conception Unit, Division of 
      Women's Health, King's College School of Medicine, London, UK.
FAU - Stephenson, Emma
AU  - Stephenson E
FAU - Collins, Robert
AU  - Collins R
FAU - Patel, Heema
AU  - Patel H
FAU - Trussler, Jane
AU  - Trussler J
FAU - Al-Bedaery, Roaa
AU  - Al-Bedaery R
FAU - Renwick, Pamela
AU  - Renwick P
FAU - Ogilvie, Caroline
AU  - Ogilvie C
FAU - Vaughan, Robert
AU  - Vaughan R
FAU - Ilic, Dusko
AU  - Ilic D
LA  - eng
GR  - 086034/WT_/Wellcome Trust/United Kingdom
GR  - G0701172/MRC_/Medical Research Council/United Kingdom
GR  - G0801061/MRC_/Medical Research Council/United Kingdom
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20121119
PL  - England
TA  - EMBO Mol Med
JT  - EMBO molecular medicine
JID - 101487380
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Cell Culture Techniques/methods/standards
MH  - Cell Line
MH  - Embryonic Stem Cells/*cytology/*immunology
MH  - Female
MH  - HLA Antigens/genetics
MH  - Haplotypes
MH  - *Histocompatibility Testing/methods
MH  - Humans
MH  - Huntington Disease/diagnosis/genetics
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Preimplantation Diagnosis/methods
MH  - Tissue Banks
PMC - PMC3569650
EDAT- 2012/11/20 06:00
MHDA- 2013/06/07 06:00
PMCR- 2013/01/01
CRDT- 2012/11/20 06:00
PHST- 2012/09/03 00:00 [received]
PHST- 2012/10/04 00:00 [revised]
PHST- 2012/10/09 00:00 [accepted]
PHST- 2012/11/20 06:00 [entrez]
PHST- 2012/11/20 06:00 [pubmed]
PHST- 2013/06/07 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.1002/emmm.201201973 [doi]
PST - ppublish
SO  - EMBO Mol Med. 2013 Jan;5(1):10-7. doi: 10.1002/emmm.201201973. Epub 2012 Nov 19.