PMID- 23161858
OWN - NLM
STAT- MEDLINE
DCOM- 20130904
LR  - 20151119
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 53
IP  - 12
DP  - 2012 Dec
TI  - PGR5-dependent cyclic electron transport around PSI contributes to the redox 
      homeostasis in chloroplasts rather than CO(2) fixation and biomass production in 
      rice.
PG  - 2117-26
LID - 10.1093/pcp/pcs153 [doi]
AB  - The PGR5 (PROTON GRADIENT REGULATION 5) gene that is required for PSI cyclic 
      electron transport in Arabidopsis was knocked down in rice (Oryza sativa). In 
      three PGR5 knockdown (KD) lines, the PGR5 protein level was reduced to 5-8% of 
      that in the wild type, resulting in a 50% reduction in PGRL1 (PGR5-LIKE 
      PHOTOSYNTHETIC PHENOTYPE 1) protein levels. In ruptured chloroplasts, 
      ferredoxin-dependent plastoquinone reduction activity was partially impaired; the 
      phenotype was mimicked by addition of antimycin A to wild-type chloroplasts. As 
      occurred in the Arabidopsis pgr5 mutant, non-photochemical quenching of Chl 
      fluorescence (NPQ) induction was impaired in the leaves, but the electron 
      transport rate (ETR) was only mildly affected at high light intensity. The 
      P700(+) level was reduced even at low light intensity, suggesting that the PGR5 
      function was severely disturbed as in the Arabidopsis pgr5 mutant and that the 
      other alternative routes of electrons could not compensate the stromal redox 
      balance. The amplitude of the light-dark electrochromic shift (ECS) signal 
      (ECSt), which reflects the total size of the proton motive force in steady-state 
      photosynthesis, was reduced by 13-25% at approximately the growth light 
      intensity. The CO(2) fixation rate was only slightly reduced in the PGR5 KD 
      lines. Despite the drastic reduction in NPQ and P700(+) levels, total biomass was 
      only slightly reduced in PGR5 KD lines grown at 370 micromol photons m(-2) s(-1). 
      These results suggest that CO(2) fixation and growth rate are very robust in the 
      face of alterations in the fundamental reactions of photosynthesis under constant 
      light conditions in rice.
FAU - Nishikawa, Yuri
AU  - Nishikawa Y
AD  - Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, 
      Kyoto, 606-8502 Japan.
FAU - Yamamoto, Hiroshi
AU  - Yamamoto H
FAU - Okegawa, Yuki
AU  - Okegawa Y
FAU - Wada, Shinya
AU  - Wada S
FAU - Sato, Nozomi
AU  - Sato N
FAU - Taira, Yoshichika
AU  - Taira Y
FAU - Sugimoto, Kazuhiko
AU  - Sugimoto K
FAU - Makino, Amane
AU  - Makino A
FAU - Shikanai, Toshiharu
AU  - Shikanai T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121118
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Ferredoxins)
RN  - 0 (PGR5 protein, Arabidopsis)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - 0 (Photosystem I Protein Complex)
RN  - 0 (Plant Proteins)
RN  - 1406-65-1 (Chlorophyll)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 642-15-9 (Antimycin A)
RN  - OAC30J69CN (Plastoquinone)
SB  - IM
MH  - Antimycin A/pharmacology
MH  - Arabidopsis/genetics/growth & development/physiology
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Biomass
MH  - Carbon Dioxide/*metabolism
MH  - Chlorophyll/metabolism
MH  - Chloroplasts/drug effects/metabolism/radiation effects
MH  - Electron Transport/radiation effects
MH  - Ferredoxins/metabolism
MH  - Gene Knockdown Techniques
MH  - Homeostasis/radiation effects
MH  - Light
MH  - Oryza/genetics/growth & development/*metabolism/radiation effects
MH  - Oxidation-Reduction/radiation effects
MH  - Phenotype
MH  - Photosynthesis/physiology
MH  - Photosynthetic Reaction Center Complex Proteins/genetics/metabolism
MH  - Photosystem I Protein Complex/*metabolism/radiation effects
MH  - Plant Leaves/genetics/growth & development/metabolism/radiation effects
MH  - Plant Proteins/genetics/*metabolism
MH  - Plant Transpiration/radiation effects
MH  - Plants, Genetically Modified
MH  - Plastoquinone/metabolism
EDAT- 2012/11/20 06:00
MHDA- 2013/09/05 06:00
CRDT- 2012/11/20 06:00
PHST- 2012/11/20 06:00 [entrez]
PHST- 2012/11/20 06:00 [pubmed]
PHST- 2013/09/05 06:00 [medline]
AID - pcs153 [pii]
AID - 10.1093/pcp/pcs153 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2012 Dec;53(12):2117-26. doi: 10.1093/pcp/pcs153. Epub 2012 
      Nov 18.