PMID- 23162640
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 4
IP  - 5
DP  - 2012 Nov
TI  - Expression of connexin 32 and connexin 43 in acute myeloid leukemia and their 
      roles in proliferation.
PG  - 1003-1007
AB  - Connexins (Cxs), a conserved family of trans-membrane proteins, function in the 
      organization of cell-cell communicatin via gap junctions in multicellular 
      organisms. However, the role of Cxs in acute myeloid leukemia (AML) is poorly 
      understood. In this study, we investigated the relationship between cell 
      proliferation and expression of connexin 43 (Cx43) and connexin 32 (Cx32) mRNA 
      and proteins in acute myeloid leukemia in vitro. Proliferation was observed using 
      a growth curve and the rate of proliferation was detected by MTT assay in the 
      acute myeloid leukemia cell lines OCI-AML3 and OCIM2. Cell cycle and cell 
      proliferation index were assessed by flow cytometry analysis. The mRNA expression 
      of the gap junction genes Cx43 and Cx32 was detected by RT-PCR. The expression of 
      Cx43 and Cx32 proteins in the cell lines was analyzed by western blot analysis 
      and immunofluorescence. The doubling time of OCI-AML3 and OCIM2 was 48 h and 36 
      h, respectively. In OCIM2, the percentage of cells in the S phase fraction was 
      59.47+/-9.6%, and the proliferation rate was 78.12+/-8.9%; however, in OCI-AML3, the 
      percentage of cells in the S phase was 24.95+/-5.8%, and the proliferation rate was 
      35.21+/-6.7%. At the mRNA level, both cell lines expressed Cx43 and Cx32, and there 
      was no significant difference in the expression of Cx43 and Cx32 mRNA in the two 
      cell lines. At the protein level, there was a significant difference in the 
      expression of Cx43, but not of Cx32. The proliferation ability of OCIM2 was 
      higher than OCI-AML3, and OCIM2 exhibited higher Cx43 western blot and 
      fluorescence intensities compared with OCI-AML3. The results suggest that a 
      higher expression of Cx43 in AML cells may play a significant role in the 
      proliferation ability.
FAU - Yi, Sha
AU  - Yi S
AD  - Department of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
FAU - Chen, Yan
AU  - Chen Y
FAU - Wen, Lu
AU  - Wen L
FAU - Yang, Lijing
AU  - Yang L
FAU - Cui, Guohui
AU  - Cui G
LA  - eng
PT  - Journal Article
DEP - 20120829
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3499603
EDAT- 2012/11/20 06:00
MHDA- 2012/11/20 06:01
PMCR- 2012/08/29
CRDT- 2012/11/20 06:00
PHST- 2012/05/15 00:00 [received]
PHST- 2012/08/08 00:00 [accepted]
PHST- 2012/11/20 06:00 [entrez]
PHST- 2012/11/20 06:00 [pubmed]
PHST- 2012/11/20 06:01 [medline]
PHST- 2012/08/29 00:00 [pmc-release]
AID - ol-04-05-1003 [pii]
AID - 10.3892/ol.2012.884 [doi]
PST - ppublish
SO  - Oncol Lett. 2012 Nov;4(5):1003-1007. doi: 10.3892/ol.2012.884. Epub 2012 Aug 29.