PMID- 23163331
OWN - NLM
STAT- MEDLINE
DCOM- 20130520
LR  - 20131121
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 28
IP  - 49
DP  - 2012 Dec 11
TI  - Biochemical-, biophysical-, and microarray-based antifungal evaluation of the 
      buffer-mediated synthesized nano zinc oxide: an in vivo and in vitro toxicity 
      study.
PG  - 16966-78
LID - 10.1021/la304120k [doi]
AB  - Here we describe a simple, novel method of zinc oxide nanoparticle (ZNP) 
      synthesis and physicochemical characterization. The dose-dependent antifungal 
      effect of ZNPs, compared to that of micronized zinc oxide (MZnO), was studied on 
      two pathogenic fungi: Aspergillus niger and Fusarium oxysporum. Superoxide 
      dismutase (SOD) activity, ascorbate peroxidase activity, catalase activity, 
      glutathione reductase (GR) activity, thiol content, lipid peroxidation, and 
      proline content in ZNP-treated fungal samples were found to be elevated in 
      comparison to the control, which strongly suggested that the antifungal effect of 
      ZNPs was due to the generation of reactive oxygen species (ROS). Protein 
      carbonylation, another marker of oxidative stress, was also evaluated by the 
      dinitrophenyl hydrazine (DNPH) binding assay and Fourier transform infrared 
      (FTIR) spectral analysis followed by Western blot and microarray analysis of 
      fungal samples to confirm ROS generation by ZNPs. Micrographic studies for the 
      morphological analysis of fungal samples (ZNP-treated and a control) exhibited an 
      alteration in fungal morphology. The bioavailability of ZNPs on fungal cell was 
      confirmed by energy-dispersive X-ray (EDX) analysis followed by high-resolution 
      transmission electron microscopy (HR-TEM) and confocal microscopic analysis of 
      the fungal samples. In vivo acute oral toxicity, acetylcholine esterase activity, 
      and a fertility study using a mice model were also investigated for ZNPs. The 
      long-term toxicity of ZNPs through intravenous injection was evaluated and 
      compared to that of MZnO. The in vitro comparative toxicity of ZNPs and MZnO was 
      evaluated on MRC-5 cells with the help of water-soluble tetrazolium (WST-1) and 
      lactate dehydrogenase (LDH) assays. These results suggested that ZNPs could be 
      used as an effective fungicide in modern medical and agricultural sciences.
FAU - Patra, Prasun
AU  - Patra P
AD  - Biological Sciences Division, Indian Statistical Institute, 203 B. T. Road, 
      Kolkata 700108, India. bioprasun@gmail.com
FAU - Mitra, Shouvik
AU  - Mitra S
FAU - Debnath, Nitai
AU  - Debnath N
FAU - Goswami, Arunava
AU  - Goswami A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121128
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Antifungal Agents)
RN  - 0 (Buffers)
RN  - 0 (Fungal Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sulfhydryl Compounds)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.1.7 (Glutathione Reductase)
RN  - SOI2LOH54Z (Zinc Oxide)
SB  - IM
MH  - Animals
MH  - Antifungal Agents/chemical synthesis/*toxicity
MH  - Aspergillus niger/drug effects/growth & development
MH  - Buffers
MH  - Catalase/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fertility/drug effects/physiology
MH  - Fungal Proteins/*metabolism
MH  - Fusarium/drug effects/growth & development
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Reductase/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Mice
MH  - Nanoparticles/chemistry/*toxicity
MH  - Oligonucleotide Array Sequence Analysis
MH  - Protein Carbonylation/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Sulfhydryl Compounds/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Zinc Oxide/chemistry/*toxicity
EDAT- 2012/11/21 06:00
MHDA- 2013/05/22 06:00
CRDT- 2012/11/21 06:00
PHST- 2012/11/21 06:00 [entrez]
PHST- 2012/11/21 06:00 [pubmed]
PHST- 2013/05/22 06:00 [medline]
AID - 10.1021/la304120k [doi]
PST - ppublish
SO  - Langmuir. 2012 Dec 11;28(49):16966-78. doi: 10.1021/la304120k. Epub 2012 Nov 28.