PMID- 23163337
OWN - NLM
STAT- MEDLINE
DCOM- 20130702
LR  - 20191210
IS  - 1365-2958 (Electronic)
IS  - 0950-382X (Linking)
VI  - 87
IP  - 1
DP  - 2013 Jan
TI  - Genetic requirements for Moraxella catarrhalis growth under iron-limiting 
      conditions.
PG  - 14-29
LID - 10.1111/mmi.12081 [doi]
AB  - Iron sequestration by the human host is a first line defence against respiratory 
      pathogens like Moraxella catarrhalis, which consequently experiences a period of 
      iron starvation during colonization. We determined the genetic requirements for 
      M. catarrhalis BBH18 growth during iron starvation using the high-throughput 
      genome-wide screening technology genomic array footprinting (GAF). By subjecting 
      a large random transposon mutant library to growth under iron-limiting 
      conditions, mutants of the MCR_0996-rhlB-yggW operon, rnd, and MCR_0457 were 
      negatively selected. Growth experiments using directed mutants confirmed the GAF 
      phenotypes with DeltayggW (putative haem-shuttling protein) and DeltaMCR_0457 
      (hypothetical protein) most severely attenuated during iron starvation, 
      phenotypes which were restored upon genetic complementation of the deleted genes. 
      Deletion of yggW resulted in similar attenuated phenotypes in three additional 
      strains. Transcriptional profiles of DeltayggW and DeltaMCR_0457 were highly altered with 
      393 and 192 differentially expressed genes respectively. In all five mutants, 
      expression of nitrate reductase genes was increased and of nitrite reductase 
      decreased, suggesting an impaired aerobic respiration. Alteration of iron 
      metabolism may affect nasopharyngeal colonization as adherence of all mutants to 
      respiratory tract epithelial cells was attenuated. In conclusion, we elucidated 
      the genetic requirements for M. catarrhalis growth during iron starvation and 
      characterized the roles of the identified genes in bacterial growth and host 
      interaction.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - de Vries, Stefan P W
AU  - de Vries SP
AD  - Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, 
      Nijmegen, the Netherlands.
FAU - Burghout, Peter
AU  - Burghout P
FAU - Langereis, Jeroen D
AU  - Langereis JD
FAU - Zomer, Aldert
AU  - Zomer A
FAU - Hermans, Peter W M
AU  - Hermans PW
FAU - Bootsma, Hester J
AU  - Bootsma HJ
LA  - eng
SI  - GEO/GSE41546
SI  - GEO/GSE41547
SI  - GEO/GSE41548
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121119
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Gene Deletion
MH  - Gene Expression Profiling
MH  - Humans
MH  - Iron/*metabolism
MH  - Microarray Analysis
MH  - Moraxella catarrhalis/genetics/*growth & development/*metabolism
MH  - Phenotype
MH  - Signal Transduction
EDAT- 2012/11/21 06:00
MHDA- 2013/07/03 06:00
CRDT- 2012/11/21 06:00
PHST- 2012/10/20 00:00 [accepted]
PHST- 2012/11/21 06:00 [entrez]
PHST- 2012/11/21 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
AID - 10.1111/mmi.12081 [doi]
PST - ppublish
SO  - Mol Microbiol. 2013 Jan;87(1):14-29. doi: 10.1111/mmi.12081. Epub 2012 Nov 19.