PMID- 23164369 OWN - NLM STAT- MEDLINE DCOM- 20131227 LR - 20161020 IS - 1469-5111 (Electronic) IS - 1461-1457 (Linking) VI - 16 IP - 4 DP - 2013 May TI - Region-specific effects on BDNF expression after contingent or non-contingent cocaine i.v. self-administration in rats. PG - 913-8 LID - 10.1017/S146114571200096X [doi] AB - Brain-derived neurotrophic factor (BDNF) dynamic changes were investigated in the medial prefrontal cortex (mPFC) and nucleus accumbens (NAc) during use and the early phases of cocaine abstinence after 14 sessions (2 h self-administration/d; 0.25 mg/0.1 ml.6 s infusion) by employing a 'yoked control-operant paradigm'. The effect on BDNF was region-specific and dependent on the withdrawal time. In the NAc, BDNF protein levels increased immediately after the last self-administration session, with a larger increase in passively cocaine-exposed rats. In the mPFC, BDNF expression was elevated 24 h after the last self-administration session, independently of how the drug was encountered. No changes were found in NAc and mPFC 7 d after the last self-administration session. Analysis of transcript levels in the mPFC indicated that action on exon I might contribute to BDNF's cortical induction. These findings indicate a finely tuned modulation of BDNF expression during use and early phases of cocaine abstinence. FAU - Fumagalli, Fabio AU - Fumagalli F AD - Centro di Neurofarmacologia, Dipartimento di Scienze Farmacologiche e Biomolecolari, Universita degli Studi di Milano, Milano, Italy. fabio.fumagalli@unimi.it FAU - Moro, Federico AU - Moro F FAU - Caffino, Lucia AU - Caffino L FAU - Orru, Alessandro AU - Orru A FAU - Cassina, Chiara AU - Cassina C FAU - Giannotti, Giuseppe AU - Giannotti G FAU - Di Clemente, Angelo AU - Di Clemente A FAU - Racagni, Giorgio AU - Racagni G FAU - Riva, Marco A AU - Riva MA FAU - Cervo, Luigi AU - Cervo L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20121120 PL - England TA - Int J Neuropsychopharmacol JT - The international journal of neuropsychopharmacology JID - 9815893 RN - 0 (Brain-Derived Neurotrophic Factor) RN - I5Y540LHVR (Cocaine) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*biosynthesis MH - Cocaine/*administration & dosage MH - *Gene Expression Regulation MH - Infusions, Intravenous MH - Male MH - Nucleus Accumbens/drug effects/*metabolism MH - Prefrontal Cortex/drug effects/*metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Self Administration EDAT- 2012/11/21 06:00 MHDA- 2013/12/29 06:00 CRDT- 2012/11/21 06:00 PHST- 2012/11/21 06:00 [entrez] PHST- 2012/11/21 06:00 [pubmed] PHST- 2013/12/29 06:00 [medline] AID - S146114571200096X [pii] AID - 10.1017/S146114571200096X [doi] PST - ppublish SO - Int J Neuropsychopharmacol. 2013 May;16(4):913-8. doi: 10.1017/S146114571200096X. Epub 2012 Nov 20.