PMID- 23164608
OWN - NLM
STAT- MEDLINE
DCOM- 20130228
LR  - 20130108
IS  - 2210-7762 (Print)
VI  - 205
IP  - 12
DP  - 2012 Dec
TI  - Localization of centromeric breaks in head and neck squamous cell carcinoma.
PG  - 622-9
LID - S2210-7762(12)00251-7 [pii]
LID - 10.1016/j.cancergen.2012.10.002 [doi]
AB  - Head and neck squamous cell carcinoma (HNSCC) have very complex karyotypes that 
      show all types of structural rearrangements. The most frequent aberrations are 
      whole-arm translocations, which appear to have their breakpoints in centromeric 
      or pericentromeric regions. We aimed to pinpoint the exact location of the 
      breakpoints of these marker chromosomes with high-resolution cytogenetic and 
      genetic analyses using microarray comparative genomic hybridization (CGH), 
      multiplex ligation-dependent probe amplification (MLPA), and fiber fluorescence 
      in situ hybridization (FISH). Among the seven cell lines in this study, six (84%) 
      harbored one or more centromeric breakpoints or whole-arm translocations. In 
      total, microarray CGH identified 163 breakpoints, 47 (29%) of which were in 
      centromeric regions. Microarray CGH and MLPA results indicated that the 
      translocation breakpoints were localized between the microarray oligonucleotide 
      clones and MLPA probes closest to the centromere. High-resolution fiber-FISH 
      revealed adjacent or minimally overlapping signals of probes that recognize the 
      pericentromeric sequences of the two participating chromosomes. This indicates 
      that whole chromosome arm translocation breakpoints occur within the 
      pericentromeric chromatin and not the centromere core sequences.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Martinez, Jorge Garcia
AU  - Martinez JG
AD  - Department of Otolaryngology, Instituto Universitario de Oncologia del Principado 
      de Asturias (IUOPA), Hospital Universitario Central de Asturias, Oviedo, 
      Asturias, Spain.
FAU - Perez-Escuredo, Jhudit
AU  - Perez-Escuredo J
FAU - Llorente, Jose Luis
AU  - Llorente JL
FAU - Suarez, Carlos
AU  - Suarez C
FAU - Hermsen, Mario A
AU  - Hermsen MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121117
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - Centromere/*genetics
MH  - *Chromosome Breakage
MH  - Chromosomes, Human/genetics
MH  - Comparative Genomic Hybridization
MH  - DNA Copy Number Variations/genetics
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Isochromosomes/genetics
MH  - Models, Biological
MH  - Multiplex Polymerase Chain Reaction
MH  - Oligonucleotide Array Sequence Analysis
MH  - Translocation, Genetic
EDAT- 2012/11/21 06:00
MHDA- 2013/03/01 06:00
CRDT- 2012/11/21 06:00
PHST- 2012/04/03 00:00 [received]
PHST- 2012/10/01 00:00 [revised]
PHST- 2012/10/02 00:00 [accepted]
PHST- 2012/11/21 06:00 [entrez]
PHST- 2012/11/21 06:00 [pubmed]
PHST- 2013/03/01 06:00 [medline]
AID - S2210-7762(12)00251-7 [pii]
AID - 10.1016/j.cancergen.2012.10.002 [doi]
PST - ppublish
SO  - Cancer Genet. 2012 Dec;205(12):622-9. doi: 10.1016/j.cancergen.2012.10.002. Epub 
      2012 Nov 17.