PMID- 23165982
OWN - NLM
STAT- MEDLINE
DCOM- 20130503
LR  - 20121127
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 31
IP  - 1
DP  - 2013 Jan
TI  - Involvement of Nrf2 activation in the upregulation of S100A9 by exposure to 
      inorganic arsenite.
PG  - 259-64
LID - 10.3892/ijmm.2012.1185 [doi]
AB  - This study examined whether S100A8 and S100A9, which comprise a complex called 
      calprotectin, are upregulated by exposure to sodium arsenite [As(III)] in nine 
      lines of human-derived cells. HaCaT skin keratinocyte cells, U937 leukemic 
      monocyte cells, and UROtsa urothelial cells showed increased mRNA levels of 
      S100A8 and S100A9 after a 2-week exposure to As(III). To understand the 
      mechanisms regulating S100A9 upregulation in response to As(III), we tested 
      S100A9 promoter-dependent luciferase activity in HaCaT cells transfected with 
      S100A9 promoter (-1000/+429)-fused luciferase cDNA. The results indicated that 
      exposure to As(III) stimulated S100A9 promoter-dependent luciferase activity. In 
      addition, the transcription NF-E2-related factor 2 (Nrf2) was strongly activated 
      in HaCaT cells exposed to As(III). Since two putative antioxidant response 
      elements were found in the S100A9 promoter (ARE1, 5'-ACAGGCAGGG-3' from -897 to 
      -887; ARE2, 5'-ATCTTCCGGAG-3' from -78 to -67), we constructed deletion mutants 
      of each ARE on S100A9 promoter-fused luciferase cDNA. The results indicated that 
      ARE2 is the responsible element for the activation of S100A9 transcription in 
      response to As(III). This report is the first to demonstrate that As(III) 
      enhanced S100A8 and S100A9 expression in human-derived cells and As(III)-induced 
      S100A9 expression is dependent on Nrf2 activation.
FAU - Sumi, Daigo
AU  - Sumi D
AD  - Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical 
      Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan.
FAU - Shimizu, Yuri
AU  - Shimizu Y
FAU - Himeno, Seiichiro
AU  - Himeno S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121116
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Arsenites)
RN  - 0 (Calgranulin A)
RN  - 0 (Calgranulin B)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Sodium Compounds)
RN  - 48OVY2OC72 (sodium arsenite)
SB  - IM
MH  - Arsenites/*toxicity
MH  - Calgranulin A/genetics/metabolism
MH  - Calgranulin B/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Epithelial Cells/drug effects/pathology
MH  - Humans
MH  - Keratinocytes/cytology/metabolism/*pathology
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Sodium Compounds/*toxicity
MH  - *Transcriptional Activation
MH  - Up-Regulation
MH  - Urothelium/drug effects/pathology
EDAT- 2012/11/21 06:00
MHDA- 2013/05/04 06:00
CRDT- 2012/11/21 06:00
PHST- 2012/08/23 00:00 [received]
PHST- 2012/10/19 00:00 [accepted]
PHST- 2012/11/21 06:00 [entrez]
PHST- 2012/11/21 06:00 [pubmed]
PHST- 2013/05/04 06:00 [medline]
AID - 10.3892/ijmm.2012.1185 [doi]
PST - ppublish
SO  - Int J Mol Med. 2013 Jan;31(1):259-64. doi: 10.3892/ijmm.2012.1185. Epub 2012 Nov 
      16.