PMID- 23167899
OWN - NLM
STAT- MEDLINE
DCOM- 20130715
LR  - 20131121
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 37
IP  - 3
DP  - 2013 Feb
TI  - Regulation of synaptic currents by mGluR2 at reciprocal synapses in the mouse 
      accessory olfactory bulb.
PG  - 351-8
LID - 10.1111/ejn.12059 [doi]
AB  - The throughput of information from the accessory olfactory bulb (AOB) to 
      downstream structures is controlled by reciprocal dendrodendritic inhibition of 
      mitral cells by granule cells. Given the high expression levels of mGluR2, a 
      metabotropic glutamate receptor, in the AOB and the fact that the activation of 
      mGluR2 permits the formation of a specific olfactory memory, we reasoned that 
      mGluR2 might play an important role in regulating dendrodendritic inhibition. To 
      test this hypothesis, we examined the effects of pharmacological and genetic 
      manipulations of mGluR2 on synaptic responses measured from mitral or granule 
      cells in slice preparations from 23- to 36-day-old Balb/c mice. To evoke 
      dendrodendritic inhibition, a depolarizing voltage step from -70 to 0 mV or a 
      threshold current stimulus adjusted to elicit action potential(s) was applied to 
      a mitral cell using either a nystatin-perforated or conventional whole-cell 
      configuration. We found that an agonist for group II metabotropic glutamate 
      receptors (mGluR2/mGluR3), DCG-IV 
      [(2S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine], suppressed, whereas the 
      mGluR2/mGluR3 antagonist LY341495 
      [(alphaS)-alpha-amino-alpha-[(1S,2S)-2-carboxycyclopropyl]-9H-xanthine-9-propanoic acid] 
      enhanced dendrodendritic inhibition. Genetic ablation of mGluR2 markedly impaired 
      the effects of DCG-IV and LY341495 on dendrodendritic inhibition. DCG-IV reduced 
      both the frequency and the amplitude of spontaneous miniature excitatory 
      postsynaptic currents recorded from granule cells. Additionally, DCG-IV inhibited 
      high-voltage-activated calcium currents in both mitral and granule cells. These 
      results suggest that mGluR2 reduces dendrodendritic inhibition by inhibiting 
      synaptic transmission between mitral cells and granule cells in the AOB.
CI  - (c) 2012 Federation of European Neuroscience Societies and Blackwell Publishing 
      Ltd.
FAU - Taniguchi, Mutsuo
AU  - Taniguchi M
AD  - Department of Physiology, Kochi Medical School, Nankoku, Japan. 
      tanigucm@kochi-u.ac.jp
FAU - Yokoi, Mineto
AU  - Yokoi M
FAU - Shinohara, Yoshiaki
AU  - Shinohara Y
FAU - Okutani, Fumino
AU  - Okutani F
FAU - Murata, Yoshihiro
AU  - Murata Y
FAU - Nakanishi, Shigetada
AU  - Nakanishi S
FAU - Kaba, Hideto
AU  - Kaba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121121
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Amino Acids)
RN  - 0 (Anticonvulsants)
RN  - 0 (Calcium Channels)
RN  - 0 (Cyclopropanes)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (LY 341495)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Xanthenes)
RN  - 0 (metabotropic glutamate receptor 2)
RN  - 147782-19-2 (2-(2,3-dicarboxycyclopropyl)glycine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Action Potentials
MH  - Amino Acids/pharmacology
MH  - Animals
MH  - Anticonvulsants/pharmacology
MH  - Calcium Channels/drug effects/metabolism
MH  - Cyclopropanes/pharmacology
MH  - Dendrites/metabolism/physiology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - *Excitatory Postsynaptic Potentials
MH  - Glycine/analogs & derivatives/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutation
MH  - Olfactory Bulb/cytology/metabolism/*physiology
MH  - Receptors, Metabotropic Glutamate/agonists/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Synapses/metabolism/*physiology
MH  - Xanthenes/pharmacology
EDAT- 2012/11/22 06:00
MHDA- 2013/07/17 06:00
CRDT- 2012/11/22 06:00
PHST- 2012/10/02 00:00 [received]
PHST- 2012/10/16 00:00 [accepted]
PHST- 2012/11/22 06:00 [entrez]
PHST- 2012/11/22 06:00 [pubmed]
PHST- 2013/07/17 06:00 [medline]
AID - 10.1111/ejn.12059 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2013 Feb;37(3):351-8. doi: 10.1111/ejn.12059. Epub 2012 Nov 21.