PMID- 23170121
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 4
IP  - 4
DP  - 2012 Oct
TI  - Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction 
      of tumor antigen-specific antitumor immunity.
PG  - 655-660
AB  - Dendritic cells (DCs) are highly efficient, specialized antigen-presenting cells 
      and DCs transfected with tumor-related antigens are regarded as promising 
      vaccines in cancer immunotherapy. The aim of the present study was to investigate 
      whether DCs co-transfected with the alpha-fetoprotein (AFP) and human interleukin-2 
      (IL-2) genes were able to induce stronger therapeutic antitumor immunity in 
      transfected DCs. In this study, DCs from hepatocellular carcinoma (HCC) patients 
      were co-transfected with the IL-2 gene and/or the AFP gene. The reverse 
      transcription-PCR (RT-PCR) data revealed that the DCs transfected with the 
      adenovirus AdAFP/IL-2 expressed AFP and IL-2. The DCs co-transfected with IL-2 
      and AFP (AFP/IL-2-DCs) enhanced the cytotoxicities of cytotoxic T lymphocytes 
      (CTLs) and increased the production of IL-2 and interferon-gamma significantly 
      compared with their AFP-DC, green fluorescent protein (GFP)-DC, DC or 
      phosphate-buffered saline (PBS) counterparts. In vivo data suggested that 
      immunization with AFP-DCs enhances antigen-specific antitumor efficacy more 
      potently than immunization with IL-2-DCs or AFP-DCs. These findings provide a 
      potential strategy to improve the efficacy of DC-based tumor vaccines.
FAU - Yang, Jing-Yue
AU  - Yang JY
AD  - Department of Clinical Oncology, State Key Discipline of Cell Biology, Xijing 
      Hospital;
FAU - Li, Xiao
AU  - Li X
FAU - Gao, Li
AU  - Gao L
FAU - Teng, Zeng-Hui
AU  - Teng ZH
FAU - Liu, Wen-Chao
AU  - Liu WC
LA  - eng
PT  - Journal Article
DEP - 20120706
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3501441
EDAT- 2012/11/22 06:00
MHDA- 2012/11/22 06:01
PMCR- 2013/10/01
CRDT- 2012/11/22 06:00
PHST- 2012/04/15 00:00 [received]
PHST- 2012/06/28 00:00 [accepted]
PHST- 2012/11/22 06:00 [entrez]
PHST- 2012/11/22 06:00 [pubmed]
PHST- 2012/11/22 06:01 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - etm-04-04-0655 [pii]
AID - 10.3892/etm.2012.635 [doi]
PST - ppublish
SO  - Exp Ther Med. 2012 Oct;4(4):655-660. doi: 10.3892/etm.2012.635. Epub 2012 Jul 6.