PMID- 23170232
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121122
LR  - 20211021
IS  - 2045-8827 (Electronic)
IS  - 2045-8827 (Print)
IS  - 2045-8827 (Linking)
VI  - 1
IP  - 3
DP  - 2012 Sep
TI  - Abundance and short-term temporal variability of fecal microbiota in healthy 
      dogs.
PG  - 340-7
LID - 10.1002/mbo3.36 [doi]
AB  - Temporal variations of intestinal microorganisms have been investigated in 
      humans, but limited information is available for other animal species. The aim of 
      the study was to evaluate the abundance and short-term temporal variability of 
      fecal microbiota in dogs. Two fecal samples were collected (15 days apart) from 
      six healthy dogs. The microbiota was evaluated using fluorescence in situ 
      hybridization (FISH) and 454-pyrosequencing targeting the 16S rRNA and its gene. 
      Pyrosequencing revealed 15 families comprising >80% of all microbiota, over time 
      intraindividual coefficients of variation (%CV) ranged from 2% to 141% (median: 
      55%). In contrast, the interindividual %CV ranged from 62% to 230% (median: 
      145%). Relative proportions of Faecalibacterium (important for intestinal health) 
      and Subdoligranulum were low (two dogs harbored 4-7% of Subdoligranulum, the 
      remaining dogs had <1% of either genus). Conversely, FISH revealed that 
      Faecalibacterium comprised a median of 5% of total counts (range: 0-8%, probe 
      Fprau645). A novel FISH probe (Faecali 698) was tested that, compared with 
      Fprau645, can detect in silico a similar percentage of Faecalibacterium but 
      higher proportions of Subdoligranulum. This probe revealed a high percentage of 
      Faecalibacterium-Subdoligranulum (median: 16% of total counts). Future studies 
      should consider the observed variability and discrepancies in microbial abundance 
      between FISH and 454-pyrosequencing.
FAU - Garcia-Mazcorro, Jose F
AU  - Garcia-Mazcorro JF
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, 
      College of Veterinary Medicine, Texas A&M University College Station, TX.
FAU - Dowd, Scot E
AU  - Dowd SE
FAU - Poulsen, Jeffrey
AU  - Poulsen J
FAU - Steiner, Jorg M
AU  - Steiner JM
FAU - Suchodolski, Jan S
AU  - Suchodolski JS
LA  - eng
PT  - Journal Article
DEP - 20120903
PL  - England
TA  - Microbiologyopen
JT  - MicrobiologyOpen
JID - 101588314
PMC - PMC3496977
OTO - NOTNLM
OT  - 454-pyrosequencing
OT  - FISH
OT  - Fecal microbiota
OT  - variability
EDAT- 2012/11/22 06:00
MHDA- 2012/11/22 06:01
PMCR- 2012/09/01
CRDT- 2012/11/22 06:00
PHST- 2012/06/15 00:00 [received]
PHST- 2012/08/07 00:00 [revised]
PHST- 2012/08/08 00:00 [accepted]
PHST- 2012/11/22 06:00 [entrez]
PHST- 2012/11/22 06:00 [pubmed]
PHST- 2012/11/22 06:01 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - 10.1002/mbo3.36 [doi]
PST - ppublish
SO  - Microbiologyopen. 2012 Sep;1(3):340-7. doi: 10.1002/mbo3.36. Epub 2012 Sep 3.