PMID- 23172800
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20131121
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 2
DP  - 2013 Feb
TI  - Sodium L-lactate differently affects brain-derived neurothrophic factor, 
      inducible nitric oxide synthase, and heat shock protein 70 kDa production in 
      human astrocytes and SH-SY5Y cultures.
PG  - 313-20
LID - 10.1002/jnr.23154 [doi]
AB  - The present study analyzed the in vitro effects induced by sodium L-lactate on 
      human astrocytes and the SH-SY5Y cell line, when added at concentrations of 5, 
      10, and 25 mmol/liter. Expression of brain-derived neurotrophic factor (BDNF), 
      inducible nitric oxide synthase (iNOS), and heat shock protein 70 kDa (HSP70) was 
      evaluated by Western blot analysis. Cell viability with MTT, release of nitric 
      oxide (NO) through the Griess reaction, and production of BDNF by enzyme-linked 
      immunoassay was determined. Data indicate that, in SH-SY5Y as well as in cortical 
      astrocytes, after 4 hr sodium L-lactate increases the expression and release of 
      BDNF, iNOS, and NO; after 24 hr, it turns is ineffective for the production of 
      the neurotrophin in SH-SY5Y and not in astrocytes, but the expression of iNOS and 
      release of NO appear to be further increased compared with those after 4 hr. 
      Sodium L-lactate influences differently the expression of HSP70 in SH-SY5Y 
      compared with astrocytes. We propose, based on these findings, that sodium 
      L-lactate affects the expression of BDNF in SH-SY5Y and astrocytes in a different 
      manner: high levels of iNOS and NO expressed in SH-SY5Y have a profound 
      inhibitory effect on the release of BDNF related to a more limited production of 
      HSP70 by SH-SY5Y. In conclusion, the results demonstrate differences in the 
      responses of SH-SY5Y and astrocytes to stimulation by high levels of sodium 
      L-lactate. Sodium L-lactate differently and dose and time dependently influences 
      the expression and release of BDNF, iNOS, NO, and HSP70 depending on the cell 
      type.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Coco, Marinella
AU  - Coco M
AD  - Department of Bio-Medical Sciences, Section of Physiology, University of Catania, 
      Catania, Italy.
FAU - Caggia, Silvia
AU  - Caggia S
FAU - Musumeci, Giuseppe
AU  - Musumeci G
FAU - Perciavalle, Vincenzo
AU  - Perciavalle V
FAU - Graziano, Adriana C E
AU  - Graziano AC
FAU - Pannuzzo, Giovanna
AU  - Pannuzzo G
FAU - Cardile, Venera
AU  - Cardile V
LA  - eng
PT  - Journal Article
DEP - 20121122
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - TU7HW0W0QT (Sodium Lactate)
SB  - IM
MH  - Astrocytes/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Dose-Response Relationship, Drug
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Neuroblastoma/pathology
MH  - Nitric Oxide Synthase Type II/*metabolism
MH  - Sodium Lactate/*pharmacology
MH  - Time Factors
EDAT- 2012/11/23 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/11/23 06:00
PHST- 2012/06/26 00:00 [received]
PHST- 2012/09/11 00:00 [revised]
PHST- 2012/09/18 00:00 [accepted]
PHST- 2012/11/23 06:00 [entrez]
PHST- 2012/11/23 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
AID - 10.1002/jnr.23154 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Feb;91(2):313-20. doi: 10.1002/jnr.23154. Epub 2012 Nov 22.