PMID- 23174900
OWN - NLM
STAT- MEDLINE
DCOM- 20130530
LR  - 20211203
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 17
IP  - 12
DP  - 2012 Nov 22
TI  - Influence of nonpolar substances on the extraction efficiency of six alkaloids in 
      Zoagumhwan investigated by ultra performance liquid chromatography and photodiode 
      array detection.
PG  - 13844-55
LID - 10.3390/molecules171213844 [doi]
AB  - A reverse phase ultra performance liquid chromatography and photodiode array 
      (UPLC-PDA) detection method was established for the determination of six 
      alkaloids in Zoagumhwan (ZGW), and further for investigating the influence of 
      nonpolar substances on the extraction efficiency of these alkaloids. The method 
      was based on a BEH C(18) (50 mm x 2.1 mm, 1.7 mum) column and mobile phase of 
      aqueous phosphoric acid and acetonitrile including 0.05% buffer solution under 
      gradient elution. ZGW samples of ZGW I, II, III and IV were obtained and prepared 
      by pre-processing the crude materials of Coptidis rhizoma and Evodiae fructus 
      using four technologies, namely direct water decoction, removal of nonpolar 
      substances in Evodiae fructus by supercritical fluid extraction (SFE), removal of 
      nonpolar substances in ZGW by SFE and removal of nonpolar substances in ZGW by 
      steam distillation. The developed and validated UPLC-PDA method was precise, 
      accurate and sensitive enough based on the facts that the six alkaloids showed 
      good regression (r > 0.9998), the limit of detections and quantifications for six 
      alkaloids were less than 28.8 and 94.5 ng/mL, respectively, and the recovery was 
      in the range of 98.56%-103.24%. The sequence of the total contents of six 
      alkaloids in these samples was ZGW II > ZGW IV > ZGW III > ZGW I. ZGW II, in 
      which nonpolar substances, including essential oils, were firstly removed from 
      Evodiae fructus by SFE, had the highest content of the total alkaloids, 
      indicating that extraction efficiency of the total alkaloids could be remarkably 
      increased after Evodiae fructus being extracted by SFE.
FAU - Zhao, Yanling
AU  - Zhao Y
AD  - China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation 
      Army, 100, the 4th Ring Road, Beijing 100039, China.
FAU - Jia, Lei
AU  - Jia L
FAU - Yang, Hongbo
AU  - Yang H
FAU - Wang, Jiabo
AU  - Wang J
FAU - Zhang, Ping
AU  - Zhang P
FAU - Li, Ruisheng
AU  - Li R
FAU - Gong, Man
AU  - Gong M
FAU - Luo, Shengqiang
AU  - Luo S
FAU - Liu, Shijing
AU  - Liu S
FAU - Xiao, Xiaohe
AU  - Xiao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121122
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Alkaloids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Oils)
RN  - CXS4LJR7EL (Coptidis rhizoma extract)
SB  - IM
MH  - *Alkaloids/chemistry/isolation & purification
MH  - Chromatography, Liquid
MH  - Coptis chinensis
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Evodia/*chemistry
MH  - Plant Oils/chemistry
PMC - PMC6268081
EDAT- 2012/11/24 06:00
MHDA- 2013/06/01 06:00
PMCR- 2012/11/22
CRDT- 2012/11/24 06:00
PHST- 2012/10/11 00:00 [received]
PHST- 2012/11/16 00:00 [revised]
PHST- 2012/11/19 00:00 [accepted]
PHST- 2012/11/24 06:00 [entrez]
PHST- 2012/11/24 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
PHST- 2012/11/22 00:00 [pmc-release]
AID - molecules171213844 [pii]
AID - molecules-17-13844 [pii]
AID - 10.3390/molecules171213844 [doi]
PST - epublish
SO  - Molecules. 2012 Nov 22;17(12):13844-55. doi: 10.3390/molecules171213844.