PMID- 23175149
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20211117
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Print)
IS  - 0007-0920 (Linking)
VI  - 108
IP  - 1
DP  - 2013 Jan 15
TI  - Brain-derived neurotrophic factor/tropomyosin-related kinase B pathway in gastric 
      cancer.
PG  - 121-30
LID - 10.1038/bjc.2012.499 [doi]
AB  - BACKGROUND: Brain-derived neutrophic factor (BDNF) is a member of the neutrophin 
      family that is known to activate the high-affinity tropomyosin-related receptor 
      kinase B (TrkB). This study aimed to clarify the clinical and biological 
      significance of the BDNF/TrkB pathway in gastric cancer. METHODS: We analysed 
      BDNF and TrkB expression in gastric cancer samples by real-time reverse 
      transcription PCR and immunohistochemistry. To investigate the biological role of 
      BDNF/TrkB axis, recombinant human BDNF (rhBDNF) and the Trk antagonist K252a were 
      used for in vitro and in vivo analysis. RESULTS: The BDNF expression at the 
      invasive front of primary tumours was significantly elevated compared with that 
      in the tumour core and adjacent normal mucosa. Increased BDNF expression at the 
      invasive front was significantly correlated with factors reflecting disease 
      progression, and poor prognosis. Increased co-expression of the BDNF/TrkB axis 
      was significantly correlated with poor prognosis. Gastric cancer cells expressed 
      BDNF, and administration of rhBDNF promoted proliferation, migration, invasion, 
      and inhibition of anoikis. These effects were generally inhibited by K252a. In an 
      in vivo assay, BDNF(+)/TrkB(+) gastric cancer cells injected into nude mice 
      established peritoneal dissemination, whereas K252a inhibited tumour growth. 
      CONCLUSION: The BDNF/TrkB pathway might be deeply involved in gastric cancer 
      disease progression.
FAU - Okugawa, Y
AU  - Okugawa Y
AD  - Department of Gastrointestinal and Pediatric Surgery, Division of Reparative 
      Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, 
      Tsu 514-8507, Japan.
FAU - Tanaka, K
AU  - Tanaka K
FAU - Inoue, Y
AU  - Inoue Y
FAU - Kawamura, M
AU  - Kawamura M
FAU - Kawamoto, A
AU  - Kawamoto A
FAU - Hiro, J
AU  - Hiro J
FAU - Saigusa, S
AU  - Saigusa S
FAU - Toiyama, Y
AU  - Toiyama Y
FAU - Ohi, M
AU  - Ohi M
FAU - Uchida, K
AU  - Uchida K
FAU - Mohri, Y
AU  - Mohri Y
FAU - Kusunoki, M
AU  - Kusunoki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121122
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Aged
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - Male
MH  - Prognosis
MH  - Receptor, trkB/*metabolism
MH  - Stomach Neoplasms/*metabolism
PMC - PMC3553513
EDAT- 2012/11/24 06:00
MHDA- 2013/03/08 06:00
PMCR- 2014/01/15
CRDT- 2012/11/24 06:00
PHST- 2012/11/24 06:00 [entrez]
PHST- 2012/11/24 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
PHST- 2014/01/15 00:00 [pmc-release]
AID - bjc2012499 [pii]
AID - 10.1038/bjc.2012.499 [doi]
PST - ppublish
SO  - Br J Cancer. 2013 Jan 15;108(1):121-30. doi: 10.1038/bjc.2012.499. Epub 2012 Nov 
      22.