PMID- 23175988
OWN - NLM
STAT- MEDLINE
DCOM- 20121220
LR  - 20221207
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 95
IP  - 5
DP  - 2012 Sep-Oct
TI  - Inclusion of 1-naphthylacetic acid and 2-(1-naphthyl)acetamide into three typical 
      multiresidue methods for LC/MS/MS analysis of tomatoes and zucchini.
PG  - 1520-7
AB  - In spite of high plant growth regulator application rates, little has been 
      reported in the literature on determination of their residues in fruits and 
      vegetables. This would be useful in monitoring good manufacturing practices and 
      overall safety through the enforcement of maximum residue levels (MRLs). The 
      present work describes method validation for the determination of 
      1-naphthylacetic acid (NAA) and 2(1-naphthyl)acetamide (NAAm) in tomato and 
      zucchini using the mini-Luke, ethyl acetate (EtOAc) and acetate-buffered quick, 
      easy, cheap, effective, rugged, and safe (QuEChERS) methods. Samples were spiked 
      at two different levels: 50 and 100 pg/kg for NAA and 20 and 100 pg/kg for NAAm. 
      These compounds were analyzed within the same chromatographic run with LC coupled 
      to triple quadrupole MS (LC/(QqQ)MS/MS) in positive and negative electrospray 
      ionization [ESI(+) and ESI(-)] modes for NAAm and NAA, respectively. For analyte 
      confirmation, LC/ESI(-)QTOF-MS was also investigated given that NAA has only one 
      multiple reaction monitoring transition (185.1-*140.9 m/z). These three common 
      methods were used to determine linearity, recoveries, precision (RSD), matrix 
      effects, repeatability, and reproducibility (n = 5) for the selected matrixes. In 
      terms of the Directorate-General for Health and Consumers (DG-SANCO) guidelines, 
      only insignificant differences were found for the multiresidue methods tested, 
      regardless of the commodity. Matrix-matched calibration was used, and LODs were 
      below 10.1 pg/kg for NAA and 6.0 pg/kg for NAAm, which were lower than the MRLs 
      established in current European Union legislation for these compounds. Obtained 
      recoveries for NAA ranged from 87 to 107% with RSD values below 10% for 
      mini-Luke, 83 to 107% with RSD <11% for EtOAc, and 76 to 85% with RSD <7% for 
      QuEChERS. NAAm recoveries ranged from 74 to 102% with RSD 5 15% for mini-Luke, 76 
      to 97% with RSD <4% for EtOAc, and 76 to 93% with RSD < 5% for QuEChERS. The 
      linearity of the response over two orders of magnitude was demonstrated (r2 > 
      0.996) for all methods used. Good repeatability and reproducibility in terms of 
      RSD were obtained (11.3-17.8%) for these analytical methods. The results were 
      acceptable within the studied ranges for both analytes in all methods. As these 
      multiresidue methods are used at the present time in many laboratories, this 
      survey indicates that these analytes should be included in their monitoring 
      scope.
FAU - Lozano, Ana
AU  - Lozano A
AD  - European Union Reference Laboratory (EURL), Pesticide Residue Research Group, 
      University of Almeria, Department of Hydrogeology and Analytical Chemistry, Ctra. 
      Sacramento S/No, La Canada de San Urbano, 04120, Almeria, Spain.
FAU - Perez-Parada, Andres
AU  - Perez-Parada A
FAU - Heinzen, Horacio
AU  - Heinzen H
FAU - Fernandez-Alba, Amadeo R
AU  - Fernandez-Alba AR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (2-(1-naphthyl)acetamide)
RN  - 0 (Naphthaleneacetic Acids)
RN  - 0 (Plant Growth Regulators)
RN  - 33T7G7757C (1-naphthaleneacetic acid)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Cucurbita/*chemistry
MH  - Food Analysis/methods
MH  - Food Contamination/*analysis
MH  - Solanum lycopersicum/*chemistry
MH  - Molecular Structure
MH  - Naphthaleneacetic Acids/*chemistry
MH  - Plant Growth Regulators/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/11/28 06:00
MHDA- 2012/12/21 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2012/12/21 06:00 [medline]
AID - 10.5740/jaoacint.11-509 [doi]
PST - ppublish
SO  - J AOAC Int. 2012 Sep-Oct;95(5):1520-7. doi: 10.5740/jaoacint.11-509.