PMID- 23176142
OWN - NLM
STAT- MEDLINE
DCOM- 20130515
LR  - 20220309
IS  - 1471-2105 (Electronic)
IS  - 1471-2105 (Linking)
VI  - 13 Suppl 16
IP  - Suppl 16
DP  - 2012
TI  - MALDI imaging mass spectrometry: statistical data analysis and current 
      computational challenges.
PG  - S11
LID - 10.1186/1471-2105-13-S16-S11 [doi]
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) imaging 
      mass spectrometry, also called MALDI-imaging, is a label-free bioanalytical 
      technique used for spatially-resolved chemical analysis of a sample. Usually, 
      MALDI-imaging is exploited for analysis of a specially prepared tissue section 
      thaw mounted onto glass slide. A tremendous development of the MALDI-imaging 
      technique has been observed during the last decade. Currently, it is one of the 
      most promising innovative measurement techniques in biochemistry and a powerful 
      and versatile tool for spatially-resolved chemical analysis of diverse sample 
      types ranging from biological and plant tissues to bio and polymer thin films. In 
      this paper, we outline computational methods for analyzing MALDI-imaging data 
      with the emphasis on multivariate statistical methods, discuss their pros and 
      cons, and give recommendations on their application. The methods of unsupervised 
      data mining as well as supervised classification methods for biomarker discovery 
      are elucidated. We also present a high-throughput computational pipeline for 
      interpretation of MALDI-imaging data using spatial segmentation. Finally, we 
      discuss current challenges associated with the statistical analysis of 
      MALDI-imaging data.
FAU - Alexandrov, Theodore
AU  - Alexandrov T
AD  - Center for Industrial Mathematics, University of Bremen, Bibliothekstr, 1, 28359 
      Bremen, Germany. theodore@uni-bremen.de
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20121105
PL  - England
TA  - BMC Bioinformatics
JT  - BMC bioinformatics
JID - 100965194
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/analysis
MH  - Cluster Analysis
MH  - Computational Biology/*methods
MH  - Data Interpretation, Statistical
MH  - Spectrometry, Mass, Matrix-Assisted Laser 
      Desorption-Ionization/*methods/*statistics & numerical data
PMC - PMC3489526
EDAT- 2012/11/28 06:00
MHDA- 2013/05/17 06:00
PMCR- 2012/11/05
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/05/17 06:00 [medline]
PHST- 2012/11/05 00:00 [pmc-release]
AID - 1471-2105-13-S16-S11 [pii]
AID - 10.1186/1471-2105-13-S16-S11 [doi]
PST - ppublish
SO  - BMC Bioinformatics. 2012;13 Suppl 16(Suppl 16):S11. doi: 
      10.1186/1471-2105-13-S16-S11. Epub 2012 Nov 5.