PMID- 23177810 OWN - NLM STAT- MEDLINE DCOM- 20140417 LR - 20121126 IS - 1773-0449 (Electronic) IS - 1156-5233 (Linking) VI - 22 IP - 1 DP - 2012 Mar TI - Microbial fermentation of cabbage by a bacterial strain of Pectobacterium atrosepticum for the production of bioactive material against Candida species. PG - 21-9 LID - 10.1016/j.mycmed.2011.11.004 [doi] AB - OBJECTIVE: The objective of this study was to produce the bioactive fermented product by the microbial fermentation of cabbage (Brassica oleracea) using a bacterial strain Pectobacterium atrosepticum which was assessed for its antimycotic efficacy against pathogenic isolates of Candida species. MATERIALS AND METHODS: An approach of microbial fermentation of cabbage using a bacterial strain P. atrosepticum was applied to obtain the bioactive fermented product. Antimycotic efficacy of bioactive fermented product of cabbage was evaluated by disc diffusion assay, minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations, cell viability assay and scanning electron microscopy (SEM) analysis. RESULTS: The bioactive fermented product (500mug/disc) revealed promising antimycotic effect against the tested Candida species as a diameter of inhibition zones (10+/-0.2 to 13+/-0.4mm) along with its MIC and MFC values, ranging from 250 to 1000 and 250 to 2000mug/ml, respectively. Exposure of 140min of bioactive fermented product exerted potential antimycotic effect on the viable counts of the tested fungal isolates with about 85 to 100% inhibitory effect. Further, the study of SEM revealed potential detrimental effect of bioactive product on the morphology of C. albicans KACC 30003 at MIC concentration. Elaborative study of GC-MS analysis conducted on bioactive fermented product of cabbage revealed transformation products present in fermented product. CONCLUSION: These results confirmed the therapeutic potential of microbially bioconverted/fermented products of cabbage for using in medicinal and pharmaceutical preparations. CI - Copyright A(c) 2011 Elsevier Masson SAS. All rights reserved. FAU - Bajpai, V K AU - Bajpai VK AD - School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea. FAU - Kang, S C AU - Kang SC FAU - Baek, K H AU - Baek KH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20111229 PL - France TA - J Mycol Med JT - Journal de mycologie medicale JID - 9425651 RN - 0 (Antifungal Agents) RN - 0 (Biological Products) SB - IM MH - Antifungal Agents/metabolism/*pharmacology MH - Biological Products/metabolism/*pharmacology MH - Bioreactors MH - Brassica/*microbiology MH - Candida/*drug effects MH - Colony Count, Microbial MH - *Fermentation MH - Humans MH - Microbial Sensitivity Tests MH - Pectobacterium/*physiology EDAT- 2012/11/28 06:00 MHDA- 2014/04/18 06:00 CRDT- 2012/11/27 06:00 PHST- 2011/08/22 00:00 [received] PHST- 2011/11/03 00:00 [revised] PHST- 2011/11/12 00:00 [accepted] PHST- 2012/11/27 06:00 [entrez] PHST- 2012/11/28 06:00 [pubmed] PHST- 2014/04/18 06:00 [medline] AID - S1156-5233(11)00145-4 [pii] AID - 10.1016/j.mycmed.2011.11.004 [doi] PST - ppublish SO - J Mycol Med. 2012 Mar;22(1):21-9. doi: 10.1016/j.mycmed.2011.11.004. Epub 2011 Dec 29.