PMID- 23178197
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20151119
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 157
IP  - 2
DP  - 2013 Mar
TI  - Evaluation of biomarker potential of cytochrome P450 1A (CYP1A) gene in the 
      marine medaka, Oryzias melastigma exposed to water-accommodated fractions (WAFs) 
      of Iranian crude oil.
PG  - 172-82
LID - S1532-0456(12)00136-6 [pii]
LID - 10.1016/j.cbpc.2012.11.003 [doi]
AB  - CYP1A is involved in the metabolism of diverse chemicals, including polycyclic 
      aromatic hydrocarbons and alkylated-PAHs, as a first line of detoxification 
      mechanism. First, we identified and characterized the CYP1A gene from the marine 
      medaka, Oryzias melastigma. O. melastigma CYP1A (Om-CYP1A) showed a high 
      similarity of motifs/domains compared to those of vertebrates in their amino acid 
      sequences. To check whether the Om-CYP1A would be inducible, we tested two strong 
      CYP1A inducers, beta-naphthoflavone (beta-NF) and benzo[alpha]pyrene (B[alpha]P), and observed 
      concentration-dependent transient expression on transcripts of Om-CYP1A for 96 h 
      over a wide range of concentrations. Om-CYP1A mRNA level was significantly 
      increased in exposure to different concentrations of beta-NF and B[alpha]P, and its 
      expression was highly transcribed within 12 h upon the exposure to low 
      concentrations of both chemicals. Inducible transcript profiles revealed that 
      Om-CYP1A would be associated with the toxicant metabolism via AhREs/DREs/XREs in 
      its promoter region. To uncover the effects of the water-accommodated fraction 
      (WAF) of crude oil on transcripts of Om-CYP1A, we measured mRNA expression of 
      Om-CYP1A towards different concentrations of WAF for 24h. As a result, WAF 
      exposure significantly increased Om-CYP1A transcripts at all concentrations as 
      well as during time-course experiments for 96 h. In this paper, we demonstrated 
      that WAF would trigger up-regulation of the CYP1A gene that would be associated 
      with the initiation of the cellular defense systems. This finding provides a 
      better understanding of the molecular mechanism of cellular protection 
      particularly that involved in the WAF-mediated cellular response in O. 
      melastigma.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Kim, Ryeo-Ok
AU  - Kim RO
AD  - Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 
      133-791, South Korea.
FAU - Kim, Bo-Mi
AU  - Kim BM
FAU - Hwang, Dae-Sik
AU  - Hwang DS
FAU - Au, Doris W T
AU  - Au DW
FAU - Jung, Jee-Hyun
AU  - Jung JH
FAU - Shim, Won Joon
AU  - Shim WJ
FAU - Leung, Kenneth M Y
AU  - Leung KM
FAU - Wu, Rudolf S S
AU  - Wu RS
FAU - Rhee, Jae-Sung
AU  - Rhee JS
FAU - Lee, Jae-Seong
AU  - Lee JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121122
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Biomarkers)
RN  - 0 (Fish Proteins)
RN  - 0 (Petroleum)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Benzo(a)pyrene/metabolism/toxicity
MH  - Biomarkers/metabolism
MH  - Cytochrome P-450 CYP1A1/*genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Fish Proteins/*genetics/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Metabolic Detoxication, Phase I
MH  - Molecular Sequence Data
MH  - Oryzias/*genetics/growth & development/metabolism
MH  - *Petroleum
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Water Pollutants, Chemical/metabolism/*toxicity
MH  - beta-Naphthoflavone/metabolism/toxicity
EDAT- 2012/11/28 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/08/10 00:00 [received]
PHST- 2012/11/05 00:00 [revised]
PHST- 2012/11/15 00:00 [accepted]
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - S1532-0456(12)00136-6 [pii]
AID - 10.1016/j.cbpc.2012.11.003 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2013 Mar;157(2):172-82. doi: 
      10.1016/j.cbpc.2012.11.003. Epub 2012 Nov 22.