PMID- 23178669
OWN - NLM
STAT- MEDLINE
DCOM- 20140130
LR  - 20240322
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 7
IP  - 3
DP  - 2013 Mar
TI  - Tackling the minority: sulfate-reducing bacteria in an archaea-dominated 
      subsurface biofilm.
PG  - 635-51
LID - 10.1038/ismej.2012.133 [doi]
AB  - Archaea are usually minor components of a microbial community and dominated by a 
      large and diverse bacterial population. In contrast, the SM1 Euryarchaeon 
      dominates a sulfidic aquifer by forming subsurface biofilms that contain a very 
      minor bacterial fraction (5%). These unique biofilms are delivered in high 
      biomass to the spring outflow that provides an outstanding window to the 
      subsurface. Despite previous attempts to understand its natural role, the 
      metabolic capacities of the SM1 Euryarchaeon remain mysterious to date. In this 
      study, we focused on the minor bacterial fraction in order to obtain insights 
      into the ecological function of the biofilm. We link phylogenetic diversity 
      information with the spatial distribution of chemical and metabolic compounds by 
      combining three different state-of-the-art methods: PhyloChip G3 DNA microarray 
      technology, fluorescence in situ hybridization (FISH) and synchrotron 
      radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy. The 
      results of PhyloChip and FISH technologies provide evidence for selective 
      enrichment of sulfate-reducing bacteria, which was confirmed by the detection of 
      bacterial dissimilatory sulfite reductase subunit B (dsrB) genes via quantitative 
      PCR and sequence-based analyses. We further established a differentiation of 
      archaeal and bacterial cells by SR-FTIR based on typical lipid and carbohydrate 
      signatures, which demonstrated a co-localization of organic sulfate, carbonated 
      mineral and bacterial signatures in the biofilm. All these results strongly 
      indicate an involvement of the SM1 euryarchaeal biofilm in the global cycles of 
      sulfur and carbon and support the hypothesis that sulfidic springs are important 
      habitats for Earth's energy cycles. Moreover, these investigations of a bacterial 
      minority in an Archaea-dominated environment are a remarkable example of the 
      great power of combining highly sensitive microarrays with label-free infrared 
      imaging.
FAU - Probst, Alexander J
AU  - Probst AJ
AD  - Institute for Microbiology and Archaea Center, University of Regensburg, 
      Regensburg, Germany.
FAU - Holman, Hoi-Ying N
AU  - Holman HY
FAU - DeSantis, Todd Z
AU  - DeSantis TZ
FAU - Andersen, Gary L
AU  - Andersen GL
FAU - Birarda, Giovanni
AU  - Birarda G
FAU - Bechtel, Hans A
AU  - Bechtel HA
FAU - Piceno, Yvette M
AU  - Piceno YM
FAU - Sonnleitner, Maria
AU  - Sonnleitner M
FAU - Venkateswaran, Kasthuri
AU  - Venkateswaran K
FAU - Moissl-Eichinger, Christine
AU  - Moissl-Eichinger C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121122
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.8.99.3 (Hydrogensulfite Reductase)
SB  - IM
MH  - Archaea/classification/genetics/metabolism/*physiology
MH  - Bacteria/classification/genetics/metabolism
MH  - *Bacterial Physiological Phenomena
MH  - *Biodiversity
MH  - *Biofilms
MH  - DNA, Bacterial/genetics
MH  - Hydrogensulfite Reductase/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Natural Springs/chemistry/*microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Spectroscopy, Fourier Transform Infrared
PMC - PMC3578563
EDAT- 2012/11/28 06:00
MHDA- 2014/01/31 06:00
PMCR- 2014/03/01
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2014/01/31 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - ismej2012133 [pii]
AID - 10.1038/ismej.2012.133 [doi]
PST - ppublish
SO  - ISME J. 2013 Mar;7(3):635-51. doi: 10.1038/ismej.2012.133. Epub 2012 Nov 22.