PMID- 23178756
OWN - NLM
STAT- MEDLINE
DCOM- 20130607
LR  - 20141120
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 180
DP  - 2013 Jan 1
TI  - Human chorionic gonadotropin-dependent up-regulation of epiregulin and 
      amphiregulin in equine and bovine follicles during the ovulatory process.
PG  - 39-47
LID - S0016-6480(12)00422-4 [pii]
LID - 10.1016/j.ygcen.2012.10.012 [doi]
AB  - Little is known about the expression and regulation of epiregulin (EREG) and 
      amphiregulin (AREG) in ovarian follicles of large monoovulatory animal species. 
      To characterize the gonadotropin-dependent regulation of EREG and AREG mRNAs in 
      equine follicles prior to ovulation, extracts were prepared from equine follicles 
      collected during estrus between 0 and 39h post-hCG and corpora lutea obtained on 
      day 8 of the estrous cycle (day 0=day of ovulation). Results from RT-PCR/Southern 
      blot analyses showed that levels of EREG and AREG mRNAs were very low in 
      follicles obtained at 0h but increased thereafter (P<0.05), with maximal levels 
      observed 33-39h post-hCG. This significant increase was observed in both 
      granulosa and theca cells. Immunohistochemistry and immunoblot analyses confirmed 
      the hCG-dependent induction of EREG protein in both cell types. RT-PCR/Southern 
      blot analyses of ADAM17, which encodes an enzyme that cleaves and releases 
      soluble bioactive EREG and AREG, showed that levels of its transcript were high 
      and remained constant throughout the period studied. Studies on the hCG-dependent 
      regulation of EREG and AREG in bovine preovulatory follicles in vivo showed that 
      the induction of both transcripts was transient, observed predominantly at 6h 
      post-hCG and localized only in granulosa cells. To characterize the effect of 
      epidermal growth factor receptor (EGFR) activation on the expression of 
      ovulation-related genes in granulosa cells of a large monoovulatory animal 
      species, primary cultures of bovine granulosa cells were established. Results 
      from RT-PCR analyses revealed that EREG and AREG mRNAs were induced by forskolin 
      treatment in vitro; but the EGFR inhibitor PD153035 suppressed the 
      forskolin-dependent induction of several ovulation-related transcripts, including 
      PTGS2, PTGER2, TNFAIP6, PGR, MMP1, VEGFA, and CTSL2 mRNAs. Moreover, these 
      transcripts were induced in granulosa cell cultures by EGF, an analog of EREG and 
      AREG. Collectively, this study identifies differences in the temporal and 
      cellular localization of EREG and AREG expression in equine and bovine 
      preovulatory follicles, and underscores the potential role of follicular EGFR 
      activation in the regulation of ovulation-regulated genes in large monoovulatory 
      species.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Sayasith, Khampoun
AU  - Sayasith K
AD  - Centre de recherche en reproduction animale and Departement de biomedecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, Canada. 
      k.sayasith@umontreal.ca
FAU - Lussier, Jacques
AU  - Lussier J
FAU - Dore, Monique
AU  - Dore M
FAU - Sirois, Jean
AU  - Sirois J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121121
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (EREG protein, human)
RN  - 0 (Epiregulin)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Chorionic Gonadotropin/*pharmacology
MH  - EGF Family of Proteins
MH  - Epidermal Growth Factor/*metabolism
MH  - Epiregulin
MH  - Female
MH  - Glycoproteins/*metabolism
MH  - Horses
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*metabolism
MH  - Ovarian Follicle/*drug effects/*metabolism
MH  - Ovulation/drug effects/genetics
EDAT- 2012/11/28 06:00
MHDA- 2013/06/08 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/07/09 00:00 [received]
PHST- 2012/10/18 00:00 [revised]
PHST- 2012/10/22 00:00 [accepted]
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/06/08 06:00 [medline]
AID - S0016-6480(12)00422-4 [pii]
AID - 10.1016/j.ygcen.2012.10.012 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2013 Jan 1;180:39-47. doi: 10.1016/j.ygcen.2012.10.012. Epub 
      2012 Nov 21.