PMID- 23178792
OWN - NLM
STAT- MEDLINE
DCOM- 20130620
LR  - 20211021
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 62
IP  - 2
DP  - 2013 Feb
TI  - Reduced apoptosis correlates with enhanced autophagy in synovial tissues of 
      rheumatoid arthritis.
PG  - 229-37
LID - 10.1007/s00011-012-0572-1 [doi]
AB  - OBJECTIVE: Defective apoptosis contributes to the massive synovial hyperplasia in 
      rheumatoid arthritis (RA), but the mechanism is largely unknown. To investigate 
      the reasons for the reduced apoptosis in RA synovium, we analyzed autophagy and 
      its relationship to apoptosis in synovial tissues from RA and osteoarthritis (OA) 
      patients. METHODS: Synovial tissues were obtained from seven RA and 12 OA 
      patients undergoing knee replacement surgery. Apoptosis was detected by the 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay and staining for p85 fragment of PolyADP-ribose polymerase (PARP). 
      Autophagy was determined by immunoblotting for the autophagic markers Beclin-1 
      and LC3. MicroRNA-30a (miR-30a), which targets Beclin-1, was measured by 
      real-time RT-PCR. The interplay between autophagy and apoptosis was determined 
      via Spearman's correlation analysis. RESULTS: In comparison with OA, the synovial 
      tissues from RA displayed decreased TUNEL-positive nuclei (P < 0.01). In 
      contrast, Beclin-1 and LC3 were overexpressed in the synovial lining layers of 
      RA, which was correlated with decreased levels of miR-30a. Moreover, there was a 
      significant reverse relationship between apoptosis and autophagy in RA synovial 
      tissues (P < 0.01 and r = -0.8937). CONCLUSION: The impaired apoptosis in RA 
      synovium might result from increased autophagy, which in turn could be due to the 
      deregulation of miRNA-30a.
FAU - Xu, Ke
AU  - Xu K
AD  - Department of Joint Surgery, Hong Hui Hospital, Xi'an Jiaotong University College 
      of Medicine, Xi'an 710054, China.
FAU - Xu, Peng
AU  - Xu P
FAU - Yao, Jian-Feng
AU  - Yao JF
FAU - Zhang, Yin-Gang
AU  - Zhang YG
FAU - Hou, Wei-Kun
AU  - Hou WK
FAU - Lu, She-Min
AU  - Lu SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121120
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BECN1 protein, human)
RN  - 0 (Beclin-1)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Microtubule-Associated Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Arthritis, Rheumatoid/*metabolism
MH  - *Autophagy
MH  - Beclin-1
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - MicroRNAs/genetics
MH  - Microtubule-Associated Proteins/metabolism
MH  - Middle Aged
MH  - Synovial Membrane/*metabolism
EDAT- 2012/11/28 06:00
MHDA- 2013/06/21 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/04/12 00:00 [received]
PHST- 2012/10/25 00:00 [accepted]
PHST- 2012/10/15 00:00 [revised]
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/06/21 06:00 [medline]
AID - 10.1007/s00011-012-0572-1 [doi]
PST - ppublish
SO  - Inflamm Res. 2013 Feb;62(2):229-37. doi: 10.1007/s00011-012-0572-1. Epub 2012 Nov 
      20.