PMID- 23178815
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20130517
IS  - 1873-734X (Electronic)
IS  - 1010-7940 (Linking)
VI  - 43
IP  - 6
DP  - 2013 Jun
TI  - Ischaemic preconditioning prevents the liver inflammatory response to lung 
      ischaemia/reperfusion in a swine lung autotransplant model.
PG  - 1194-201
LID - 10.1093/ejcts/ezs599 [doi]
AB  - OBJECTIVES: Lung ischaemia/reperfusion (IR) induces a systemic inflammatory 
      response that causes damage to remote organs. The liver is particularly sensitive 
      to circulating inflammatory mediators that occur after IR of remote organs. 
      Recently, remote ischaemic preconditioning has been proposed as a surgical tool 
      to protect several organs from IR. The present study was designed to investigate 
      a possible protective effect of lung ischaemic preconditioning (IP) against the 
      liver inflammatory response to lung IR. METHODS: Two groups [IP and control 
      (CON)] of 10 Large White pigs underwent lung autotransplants (left pneumonectomy, 
      ex situ cranial lobectomy and caudal lobe reimplantation). Before pneumonectomy 
      was performed in the study group, IP was induced with two 5-min cycles of left 
      pulmonary arterial occlusion and a 5-min interval of reperfusion between the two 
      occlusions. Five animals underwent sham surgery. Liver biopsies were obtained 
      during surgery at (i) prepneumonectomy, (ii) prereperfusion, (iii) 10 min after 
      reperfusion of the implanted lobe and (iv) 30 min after reperfusion. The 
      expression of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1, IL-10 and 
      inducible form of nitric oxide synthase (iNOS) was analysed by western blotting. 
      The expression of mRNA for TNF-alpha, IL1, IL-10, monocyte chemoattractant protein-1 
      (MCP-1), nuclear factor kappa beta and iNOS was analysed by reverse 
      transcription-polymerase chain reaction. Caspase-3 activity was determined by 
      enzyme-linked immunosorbent assay. Non-parametric tests were used to compare 
      differences between and within groups. RESULTS: Lung IR markedly increased 
      expression of TNF-alpha (P = 0.0051) and IL-1 (P = 0.0051) and caspase-3 activity (P 
      = 0.0043) in the CON group compared with the prepneumonectomy levels. A decrease 
      of IL-10 mRNA expression was observed in the CON group after lung reperfusion. In 
      the IP group, TNF-alpha (P = 0.0011) and IL-1 (P = 0.0001) expression and caspase-3 
      activity (P < 0.0009) were lower after reperfusion than in the CON group. IP 
      caused reversion of the observed decrease of IL-10 mRNA expression (P = 0.016) 
      induced in liver tissue by lung IR. Lung IR markedly increased the expression of 
      mRNA MCP-1 after 10 min (P = 0.0051) and 30 min (P = 0.0051) of reperfusion. 
      These increases were not observed in the IP or sham groups. CONCLUSIONS: IP 
      prevented liver injury induced by lung IR through the reduction of 
      proinflammatory cytokines and hepatocyte apoptosis.
FAU - Huerta, Luis
AU  - Huerta L
AD  - Department of Thoracic Surgery, Gregorio Maranon University General Hospital, 
      Madrid, Spain. luishuema@hotmail.com
FAU - Rancan, Lisa
AU  - Rancan L
FAU - Simon, Carlos
AU  - Simon C
FAU - Isea, Jesus
AU  - Isea J
FAU - Vidaurre, Eduardo
AU  - Vidaurre E
FAU - Vara, Elena
AU  - Vara E
FAU - Garutti, Ignacio
AU  - Garutti I
FAU - Gonzalez-Aragoneses, Federico
AU  - Gonzalez-Aragoneses F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121123
PL  - Germany
TA  - Eur J Cardiothorac Surg
JT  - European journal of cardio-thoracic surgery : official journal of the European 
      Association for Cardio-thoracic Surgery
JID - 8804069
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cytokines/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Inflammation/*metabolism
MH  - Ischemic Preconditioning/*methods
MH  - Liver Diseases/*etiology/metabolism
MH  - Lung Transplantation/*methods
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Pneumonectomy
MH  - Random Allocation
MH  - Reperfusion Injury/*metabolism/*prevention & control
MH  - Statistics, Nonparametric
MH  - Swine
OTO - NOTNLM
OT  - Ischaemia/reperfusion injury
OT  - Ischaemic preconditioning
OT  - Liver
OT  - Lung
EDAT- 2012/11/28 06:00
MHDA- 2014/03/07 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - ezs599 [pii]
AID - 10.1093/ejcts/ezs599 [doi]
PST - ppublish
SO  - Eur J Cardiothorac Surg. 2013 Jun;43(6):1194-201. doi: 10.1093/ejcts/ezs599. Epub 
      2012 Nov 23.