PMID- 23178856
OWN - NLM
STAT- MEDLINE
DCOM- 20130705
LR  - 20161125
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 45
IP  - 2
DP  - 2013 Feb
TI  - Interleukin-18 augments growth ability of primary human melanocytes by PTEN 
      inactivation through the AKT/NF-kappaB pathway.
PG  - 308-16
LID - S1357-2725(12)00371-8 [pii]
LID - 10.1016/j.biocel.2012.11.008 [doi]
AB  - Normal human skin relies on melanocytes to provide photoprotection and 
      thermoregulation by producing melanin. The growth and behavior of melanocytes are 
      controlled by many factors. Interleukin-18 (IL-18) is expressed in both immune 
      and non-immune cells and participates in the adjustment of multitude cellular 
      functions. Nonetheless, the regulative roles of IL-18 in melanogenesis and growth 
      of melanocytes have not been explored. The present study was conducted to 
      investigate the effects of IL-18 on melanocytes and elucidate the underlying 
      mechanisms. We proved that IL-18 increased the tyrosinase activity and melanin 
      content in normal human foreskin-derived epidermal melanocytes (NHEM). Treatment 
      with IL-18 (20 ng/ml) enhanced the expression of c-Kit, microphtalmia-associated 
      transcription factor (MITF) and its downstream tyrosinase-related protein 1 
      (TRP-1), and TRP-2. In addition, IL-18 induced NHEM migration at concentration of 
      20 ng/ml. These results indicated a promotive action of IL-18 on melanogenesis in 
      NHEM. Our data revealed that IL-18 stimulated ERK1/2 and NF-kappaB activation, 
      improved p-Akt, p70 S6K and anti-apoptotic Bcl-2 levels, and deactivated 
      phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in NHEM. Besides, 
      IL-18 increased level of PTEN phosphorylation to protect NHEM from damage induced 
      by H(2)O(2). These results in vitro showed the accommodation of IL-18 in 
      melanocytes growth. Therefore, we suggested an important regulating action of 
      IL-18 to melanogenesis and cell growth ability of skin melanocytes.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Zhou, Jia
AU  - Zhou J
AD  - New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, 
      China.
FAU - Shang, Jing
AU  - Shang J
FAU - Song, Jing
AU  - Song J
FAU - Ping, Fengfeng
AU  - Ping F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121122
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Interleukin-18)
RN  - 0 (MITF protein, human)
RN  - 0 (Melanins)
RN  - 0 (Microphthalmia-Associated Transcription Factor)
RN  - 0 (NF-kappa B)
RN  - 0 (Oxidants)
RN  - 0 (Recombinant Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
SB  - IM
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Foreskin/cytology
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Interleukin-18/pharmacology/*physiology
MH  - Intramolecular Oxidoreductases/metabolism
MH  - Male
MH  - Melanins/biosynthesis
MH  - Melanocytes/*physiology
MH  - Microphthalmia-Associated Transcription Factor/metabolism
MH  - NF-kappa B/metabolism
MH  - Oxidants/pharmacology
MH  - Oxidoreductases/metabolism
MH  - PTEN Phosphohydrolase/*metabolism
MH  - Phosphorylation
MH  - Primary Cell Culture
MH  - Protein Processing, Post-Translational
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Proto-Oncogene Proteins c-kit/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - *Signal Transduction
EDAT- 2012/11/28 06:00
MHDA- 2013/07/06 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/09/09 00:00 [received]
PHST- 2012/11/08 00:00 [revised]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/07/06 06:00 [medline]
AID - S1357-2725(12)00371-8 [pii]
AID - 10.1016/j.biocel.2012.11.008 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2013 Feb;45(2):308-16. doi: 
      10.1016/j.biocel.2012.11.008. Epub 2012 Nov 22.