PMID- 23179209
OWN - NLM
STAT- MEDLINE
DCOM- 20140319
LR  - 20211021
IS  - 1432-1963 (Electronic)
IS  - 0172-8113 (Linking)
VI  - 34
IP  - 4
DP  - 2013 Jul
TI  - [Round robin test for detection of genomic aberrations in non-Hodgkin lymphoma by 
      in situ hybridization].
PG  - 329-34
LID - 10.1007/s00292-012-1719-0 [doi]
AB  - BACKGROUND: The detection of characteristic genomic aberrations by fluorescence 
      in situ hybridization (FISH) has a high diagnostic impact on lymphomas according 
      to the World Health Organization (WHO). To investigate the reproducibility of 
      non-isotopic ISH results a multicenter trial was carried out involving eight 
      institutes for hematopathology. MATERIAL AND METHODS: Analyses were performed on 
      two diffuse large B-cell lymphomas (DLBCL) without known aberrations, on one 
      follicular lymphoma with a IGH/BCL2 translocation and BCL6 split and on two 
      B-cell lymphomas intermediate between DLBCL and Burkitt's lymphoma with c-MYC and 
      BCL2 rearrangements, one with an additional BCL6 split. Break-apart probes for 
      BCL6 and c-MYC, as well as fusion probes for the c-MYC/IGH and the IGH/BCL2 
      translocations were used. RESULTS: All aberrations were correctly detected by all 
      centres and no false positive or false negative results were obtained. The 
      numbers of positive cells varied from 25% to 94%. Pearson's correlation 
      coefficient between the centres was always > 0.8. CONCLUSIONS: The ISH analysis 
      of recurrent genomic aberrations in formalin-fixed paraffin-embedded (FFPE) 
      tissue is a highly reproducible technique which yields substantial additive help 
      for lymphoma diagnostics.
FAU - Barth, T F E
AU  - Barth TF
AD  - Institut fur Pathologie, Universitatsklinikum Ulm, Albert-Einstein-Allee 23, Ulm, 
      Germany. thomas.barth@uniklinik-ulm.de
FAU - Flossbach, L
AU  - Flossbach L
FAU - Bernd, H-W
AU  - Bernd HW
FAU - Bob, R
AU  - Bob R
FAU - Buck, M
AU  - Buck M
FAU - Cogliatti, S B
AU  - Cogliatti SB
FAU - Feller, A C
AU  - Feller AC
FAU - Hansmann, M L
AU  - Hansmann ML
FAU - Hartmann, S
AU  - Hartmann S
FAU - Horn, H
AU  - Horn H
FAU - Klapper, W
AU  - Klapper W
FAU - Kradolfer, D
AU  - Kradolfer D
FAU - Mattfeldt, T
AU  - Mattfeldt T
FAU - Moller, P
AU  - Moller P
FAU - Rosenwald, A
AU  - Rosenwald A
FAU - Stein, H
AU  - Stein H
FAU - Thorns, C
AU  - Thorns C
FAU - Ott, G
AU  - Ott G
LA  - ger
PT  - Comparative Study
PT  - Journal Article
TT  - Ringversuch zum Nachweis genomischer Veranderungen bei Non-Hodgkin-Lymphomen 
      mittels In-situ-Hybridisierung.
PL  - Germany
TA  - Pathologe
JT  - Der Pathologe
JID - 8006541
RN  - 0 (BCL6 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - Burkitt Lymphoma/diagnosis/genetics/pathology
MH  - *Chromosome Aberrations
MH  - DNA-Binding Proteins/genetics
MH  - Diagnosis, Differential
MH  - Genes, myc/genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lymphoma, Follicular/diagnosis/genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/diagnosis/genetics/pathology
MH  - Lymphoma, Non-Hodgkin/diagnosis/*genetics/pathology
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Quality Assurance, Health Care
MH  - Reproducibility of Results
MH  - Translocation, Genetic/genetics
EDAT- 2012/11/28 06:00
MHDA- 2014/03/22 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
AID - 10.1007/s00292-012-1719-0 [doi]
PST - ppublish
SO  - Pathologe. 2013 Jul;34(4):329-34. doi: 10.1007/s00292-012-1719-0.