PMID- 23179825
OWN - NLM
STAT- MEDLINE
DCOM- 20130502
LR  - 20121126
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 946
DP  - 2013
TI  - Classical and molecular cytogenetic analysis.
PG  - 39-60
LID - 10.1007/978-1-62703-128-8_4 [doi]
AB  - Cytogenetic analysis is performed on cell cultures for several reasons, notably, 
      to perform identity checks by verifying species of origin or the retention of key 
      chromosome rearrangements in cell lines described previously. De novo chromosome 
      analysis is usually performed when characterizing cancer cell lines for the 
      presence of neoplastic rearrangements associated with specific tumors. This 
      usually involves fluorescence in situ hybridization (FISH) using clones covering 
      gene loci near recurrent chromosome breakpoints. Chromosome breakage is an 
      important endpoint in radiation biology and mutagenesis, enabling cell lines to 
      be used for measuring genotoxic dosage and repair. Finally, cytogenetic analysis 
      may be performed to monitor stability in culture. Unlike most preparative 
      techniques, chromosome preparation resists standardization. Hence, procedures 
      must be optimized for each cell line. Thus, evidence-based protocols are 
      described for hypotonic harvesting, rapid G-banding, FISH, and Spectral 
      Karyotyping (SKY) analysis of cell cultures to allow troubleshooting and 
      fine-tuning to suit the requirements of individual cell lines.
FAU - MacLeod, Roderick A F
AU  - MacLeod RA
AD  - Department of Human and Animal Cell Cultures, DSMZ-German Collection of 
      Microorganisms and Cell Cultures, Braunschweig, Germany. rml@dsmz.de
FAU - Drexler, Hans G
AU  - Drexler HG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cells, Cultured
MH  - Chromosome Banding
MH  - Cytogenetic Analysis/*methods
MH  - Endothelial Cells/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 2012/11/28 06:00
MHDA- 2013/05/03 06:00
CRDT- 2012/11/27 06:00
PHST- 2012/11/27 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/05/03 06:00 [medline]
AID - 10.1007/978-1-62703-128-8_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;946:39-60. doi: 10.1007/978-1-62703-128-8_4.