PMID- 23180485 OWN - NLM STAT- MEDLINE DCOM- 20130703 LR - 20211021 IS - 1572-994X (Electronic) IS - 0920-8569 (Linking) VI - 46 IP - 1 DP - 2013 Feb TI - Sequence analysis of NSP9 gene of 25 PRRSV strains from Guangdong province, subtropical southern China. PG - 88-96 LID - 10.1007/s11262-012-0842-6 [doi] AB - In order to evaluate the trend in the prevalence of porcine reproductive and respiratory syndrome on farms, 25 porcine reproductive and respiratory syndrome virus (PRRSV) strains were collected from 2009 to 2012 from 11 districts in the Guangdong province. The complete gene sequences of NSP9 from the 25 PRRSV strains were amplified, sequenced, and then compared with the published sequences of other strains. The results showed that these sequences shared 97.6-99.4 % of the same nucleotides and 97.5-99.4 % of the same amino acid sequence identities with JXA1, 91.4-93.1 % of the same nucleotides and 95.4-92.6 % of the same amino acid sequence identities with VR2332, and 95.1-98.4 % of the same nucleotides and 95.3-97.0 % of the same amino acid sequences with the classical Chinese vaccine strain Ch-1a. Phylogenetic analyses showed that all of these 25 strains belonged to the North American genotype and were further divided into three sub-genotypes. This basic data allowed us to evaluate the prevalence of PRRS in the Guangdong province and the variation and evolution of the nsp9 gene in PRRSV. FAU - Zhao, Mengmeng AU - Zhao M AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, People's Republic of China. FAU - Ning, Zhangyong AU - Ning Z FAU - Wang, Heng AU - Wang H FAU - Huang, Zhen AU - Huang Z FAU - Zhang, Minze AU - Zhang M FAU - Zhang, Guihong AU - Zhang G LA - eng SI - GENBANK/JX162088 SI - GENBANK/JX162089 SI - GENBANK/JX162090 SI - GENBANK/JX162091 SI - GENBANK/JX162092 SI - GENBANK/JX162093 SI - GENBANK/JX162094 SI - GENBANK/JX162095 SI - GENBANK/JX162096 SI - GENBANK/JX162097 SI - GENBANK/JX162098 SI - GENBANK/JX162099 SI - GENBANK/JX826373 SI - GENBANK/JX826374 SI - GENBANK/JX826375 SI - GENBANK/JX826376 SI - GENBANK/JX826377 SI - GENBANK/JX826378 SI - GENBANK/JX826379 SI - GENBANK/JX826380 SI - GENBANK/JX826381 SI - GENBANK/JX826382 SI - GENBANK/JX826383 SI - GENBANK/JX826384 SI - GENBANK/JX826385 PT - Journal Article PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (RNA, Viral) RN - 0 (Viral Nonstructural Proteins) SB - IM MH - Animals MH - China MH - Cluster Analysis MH - Genotype MH - Molecular Sequence Data MH - Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/*virology MH - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & purification MH - RNA, Viral/*genetics MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid MH - Sequence Homology, Nucleic Acid MH - Swine MH - Viral Nonstructural Proteins/*genetics EDAT- 2012/11/28 06:00 MHDA- 2013/07/05 06:00 CRDT- 2012/11/27 06:00 PHST- 2012/11/27 06:00 [entrez] PHST- 2012/11/28 06:00 [pubmed] PHST- 2013/07/05 06:00 [medline] AID - 10.1007/s11262-012-0842-6 [doi] PST - ppublish SO - Virus Genes. 2013 Feb;46(1):88-96. doi: 10.1007/s11262-012-0842-6.