PMID- 23180621
OWN - NLM
STAT- MEDLINE
DCOM- 20130823
LR  - 20161125
IS  - 1097-0134 (Electronic)
IS  - 0887-3585 (Linking)
VI  - 81
IP  - 4
DP  - 2013 Apr
TI  - Salvianolic acid B inhibits the amyloid formation of human islet amyloid 
      polypeptide and protects pancreatic beta-cells against cytotoxicity.
PG  - 613-21
LID - 10.1002/prot.24216 [doi]
AB  - The misfolding of human islet amyloid polypeptide (hIAPP) is regarded as one of 
      the causative factors of type 2 diabetes mellitus (T2DM). Salvia miltiorrhiza 
      (Danshen), one of the most commonly used of traditional Chinese medicines, is 
      often used in Compound Recipes for treating diabetes, however with unclear 
      mechanisms. Since salvianolic acid B (SalB) is the most abundant bioactive 
      ingredient of salvia miltiorrhiza water-extract. In this study, we tested whether 
      SalB has any effect on the amyloidogenicity of hIAPP. Our results clearly suggest 
      that SalB can significantly inhibit the formation of hIAPP amyloid and 
      disaggregate hIAPP fibrils. Furthermore, photo-crosslinking based oligomerization 
      studies suggest SalB significantly suppresses the toxic oligomerization of hIAPP 
      monomers. Cytotoxicity protection effects on pancreatic INS-1 cells by SalB were 
      also observed using MTT-based assays, potentially due to the inhibition on the 
      membrane disruption effects and attenuated mitochondria impairment induced by 
      hIAPP. These results provide evidence that SalB may further be studied on the 
      possible pharmacological treatment for T2DM.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Cheng, Biao
AU  - Cheng B
AD  - Department of Bio-pharmaceutics, Tongji School of Pharmacy, Huazhong University 
      of Science and Technology, Wuhan, Hubei, People's Republic of China.
FAU - Gong, Hao
AU  - Gong H
FAU - Li, Xiaochao
AU  - Li X
FAU - Sun, Yue
AU  - Sun Y
FAU - Chen, Hong
AU  - Chen H
FAU - Zhang, Xin
AU  - Zhang X
FAU - Wu, Qian
AU  - Wu Q
FAU - Zheng, Ling
AU  - Zheng L
FAU - Huang, Kun
AU  - Huang K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121224
PL  - United States
TA  - Proteins
JT  - Proteins
JID - 8700181
RN  - 0 (Amyloid)
RN  - 0 (Benzofurans)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Islet Amyloid Polypeptide)
RN  - C1GQ844199 (salvianolic acid B)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amyloid/antagonists & inhibitors/chemistry/*metabolism/toxicity
MH  - Benzofurans/*pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Diabetes Mellitus, Type 2/drug therapy/metabolism/pathology
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Erythrocytes/drug effects/pathology
MH  - Hemolysis/drug effects
MH  - Humans
MH  - Insulin-Secreting Cells/*drug effects/metabolism/*pathology
MH  - Islet Amyloid Polypeptide/antagonists & inhibitors/chemistry/*metabolism/toxicity
MH  - Mitochondria/drug effects/metabolism/pathology
MH  - Molecular Sequence Data
MH  - Protein Structure, Secondary
MH  - Salvia miltiorrhiza/chemistry
EDAT- 2012/11/28 06:00
MHDA- 2013/08/24 06:00
CRDT- 2012/11/28 06:00
PHST- 2012/08/18 00:00 [received]
PHST- 2012/10/22 00:00 [revised]
PHST- 2012/11/06 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/08/24 06:00 [medline]
AID - 10.1002/prot.24216 [doi]
PST - ppublish
SO  - Proteins. 2013 Apr;81(4):613-21. doi: 10.1002/prot.24216. Epub 2012 Dec 24.