PMID- 23181102
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 4
IP  - 3
DP  - 2012 Sep
TI  - Inhibition of hepatic stellate cell proliferation by bone marrow mesenchymal stem 
      cells via regulation of the cell cycle in rat.
PG  - 375-380
AB  - The present study aimed to observe the effect of rat bone marrow mesenchymal stem 
      cells (MSCs) in vitro on hepatic stellate cell (HSC) RhoA signaling factors and 
      the expression of the cell cycle regulators P27 and cyclin D1. Rat HSC-T6 and 
      fibroblast cells were divided into control, negative control and MSC experimental 
      groups. The cell proliferation rate was examined using the WST8 assay. The cell 
      cycle was analyzed using flow cytometry. RT-PCR and western blot analysis were 
      used to examine cyclin in D1 (cyclin D1), RhoA and P27 mRNA and protein 
      expression in HSCs. After 12 h of co-culture, transition of the MSCs from the 
      G0/G1 to S phase was blocked by HSCs. In the MSC experimental group, the RhoA 
      mRNA and RhoA protein expression showed a decreasing trend with time, which was 
      statistically significant compared with that in the control and negative control 
      groups. MSC P27 protein expression showed an increasing trend with time. RhoA and 
      P27 expression were significantly negatively correlated. After 24 h of 
      co-culture, MSCs inhibited cyclin D1 expression. The difference was statistically 
      significant in the experimental and control groups as well as in the negative 
      control group (P<0.01). In conclusion, co-culture of HSCs with MSCs is capable of 
      inhibiting HSC proliferation, promoting apoptosis and inhibiting RhoA expression. 
      Reduced RhoA activity may induce an upregulation in P27 protein expression in 
      HSCs, which promotes the inhibition of cyclin D1 by MSCs and induces cell cycle 
      arrest at the G0/G1 phase, indicating a role in inhibiting rat HSC proliferation.
FAU - Qin, Shanyu
AU  - Qin S
AD  - Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530021, P.R. China.
FAU - Jiang, Haixing
AU  - Jiang H
FAU - Su, Sibiao
AU  - Su S
FAU - Wang, Dongxu
AU  - Wang D
FAU - Liang, Ziyu
AU  - Liang Z
FAU - Zhang, Junhong
AU  - Zhang J
FAU - Yang, Wen
AU  - Yang W
LA  - eng
PT  - Journal Article
DEP - 20120629
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3503536
EDAT- 2012/11/28 06:00
MHDA- 2012/11/28 06:01
PMCR- 2013/09/01
CRDT- 2012/11/28 06:00
PHST- 2012/02/12 00:00 [received]
PHST- 2012/04/24 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2012/11/28 06:01 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - etm-04-03-0375 [pii]
AID - 10.3892/etm.2012.628 [doi]
PST - ppublish
SO  - Exp Ther Med. 2012 Sep;4(3):375-380. doi: 10.3892/etm.2012.628. Epub 2012 Jun 29.