PMID- 23182962
OWN - NLM
STAT- MEDLINE
DCOM- 20130228
LR  - 20130108
IS  - 2210-7762 (Print)
VI  - 205
IP  - 12
DP  - 2012 Dec
TI  - Asynchronous DNA replication and aneuploidy in lymphocytes of hepatocellular 
      carcinoma patients.
PG  - 636-43
LID - S2210-7762(12)00256-6 [pii]
LID - 10.1016/j.cancergen.2012.10.006 [doi]
AB  - Using fluorescence in situ hybridization (FISH) analysis, we examined the 
      replication mode of the centromere region (homologous counterpart) and the 
      aneuploidy level of chromosome 17 in the interphase nuclei of phytohaemagglutinin 
      (PHA)-stimulated peripheral blood lymphocytes from (1) patients with 
      hepatocellular carcinoma (HCC); (2) patients with liver cirrhosis (LC) due to 
      hepatitis C viral infection who are individuals at a higher increased risk for 
      HCC; and (3) healthy control participants. We also compared the 
      allelic-replication asynchrony and aneuploidy frequencies with serum 
      alpha-fetoprotein (AFP) levels. We found a significant increase in centromeric 
      replication asynchrony accompanied by a high frequency of aneuploidy in 
      lymphocytes of HCC patients compared with those of LC patients and healthy 
      control participants. These changes are similar to those previously observed in 
      other types of malignancy (hematological, ovarian, prostate, and breast cancer). 
      The cytogenetic alterations of aneuploidy and strong asynchronous replication 
      displayed in the lymphocytes of HCC patients arose from malignancy, as they were 
      associated neither with an increased risk for cancer nor with an infection. The 
      cytogenetic cancer-associated markers observed in patients' lymphocytes appeared 
      to be superior to serum AFP, the marker currently used for HCC. Thus, the 
      cytogenetic cancer-associated markers may be potentially useful in noninvasive 
      cancer detection.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Hanna, Mariam Onsy F
AU  - Hanna MO
AD  - Department of Clinical Pathology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt. mariamhanna@hotmail.com
FAU - Zayed, Naglaa A
AU  - Zayed NA
FAU - Darwish, Hatem
AU  - Darwish H
FAU - Girgis, Samia I
AU  - Girgis SI
LA  - eng
PT  - Journal Article
DEP - 20121123
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - *Aneuploidy
MH  - Base Sequence
MH  - Carcinoma, Hepatocellular/blood/*genetics/*pathology
MH  - Case-Control Studies
MH  - Cell Nucleus/genetics
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA Replication/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Liver Neoplasms/blood/*genetics/*pathology
MH  - Lymphocytes/*pathology
MH  - Middle Aged
MH  - ROC Curve
MH  - Sensitivity and Specificity
MH  - alpha-Fetoproteins/metabolism
EDAT- 2012/11/28 06:00
MHDA- 2013/03/01 06:00
CRDT- 2012/11/28 06:00
PHST- 2012/02/18 00:00 [received]
PHST- 2012/09/23 00:00 [revised]
PHST- 2012/10/10 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/03/01 06:00 [medline]
AID - S2210-7762(12)00256-6 [pii]
AID - 10.1016/j.cancergen.2012.10.006 [doi]
PST - ppublish
SO  - Cancer Genet. 2012 Dec;205(12):636-43. doi: 10.1016/j.cancergen.2012.10.006. Epub 
      2012 Nov 23.