PMID- 23183272
OWN - NLM
STAT- MEDLINE
DCOM- 20130606
LR  - 20181202
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 160
IP  - 4
DP  - 2013
TI  - Fluoroquinolone photodegradation influences specific basophil activation.
PG  - 377-82
LID - 10.1159/000343023 [doi]
AB  - Fluoroquinolones (FQs) are photoreactive drugs, but it is not known whether 
      laboratory light exposure can influence the induction of photoproducts and modify 
      in vitro test results. The basophil activation test (BAT) has proven to be useful 
      for evaluating immunoglobulin E (IgE)-mediated hypersensitivity to FQs, with a 
      higher percentage of positive responders with ciprofloxacin (CIP) than with 
      moxifloxacin (MOX). We studied the effect of laboratory light on CIP and MOX 
      degradation, and drug-protein conjugate formation, and its influence on the BAT 
      for evaluating IgE-mediated hypersensitivity to FQs. The results showed an 
      important decrease in fluorescence emission intensity under light compared to 
      dark conditions for MOX, and that BAT positivity was lower in light (17.9%) than 
      in dark (35.7%) conditions. No changes were found for CIP in either fluorescence 
      emission intensity or BAT results (46.4% in both conditions). We can conclude 
      that light exposure is a critical factor in BAT results when photolabile drugs 
      like MOX are used. Therefore, light is important when interpreting in vitro 
      results.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Mayorga, Cristobalina
AU  - Mayorga C
AD  - Research Laboratory, Carlos Haya Hospital, Malaga, Spain.
FAU - Andreu, Inmaculada
AU  - Andreu I
FAU - Aranda, Ana
AU  - Aranda A
FAU - Dona, Inmaculada
AU  - Dona I
FAU - Montanez, M Isabel
AU  - Montanez MI
FAU - Blanca-Lopez, Natalia
AU  - Blanca-Lopez N
FAU - Ariza, Adriana
AU  - Ariza A
FAU - Nuin, Edurne
AU  - Nuin E
FAU - Blanca, Miguel
AU  - Blanca M
FAU - Miranda, Miguel A
AU  - Miranda MA
FAU - Torres, M Jose
AU  - Torres MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121122
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Aza Compounds)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Quinolines)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - U188XYD42P (Moxifloxacin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aza Compounds/chemistry
MH  - Basophils/*immunology
MH  - Ciprofloxacin/chemistry
MH  - Fluoroquinolones/*chemistry
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Immunoglobulin E/immunology
MH  - Light
MH  - Middle Aged
MH  - Moxifloxacin
MH  - *Photolysis
MH  - Quinolines/chemistry
MH  - Young Adult
EDAT- 2012/11/28 06:00
MHDA- 2013/06/07 06:00
CRDT- 2012/11/28 06:00
PHST- 2012/03/26 00:00 [received]
PHST- 2012/08/27 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/06/07 06:00 [medline]
AID - 000343023 [pii]
AID - 10.1159/000343023 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2013;160(4):377-82. doi: 10.1159/000343023. Epub 2012 
      Nov 22.