PMID- 23184839
OWN - NLM
STAT- MEDLINE
DCOM- 20130625
LR  - 20201209
IS  - 2192-2640 (Print)
IS  - 2192-2640 (Linking)
VI  - 2
IP  - 3
DP  - 2013 Mar
TI  - PEGylated peptide based reductive polycations as efficient nonviral gene vectors.
PG  - 481-9
LID - 10.1002/adhm.201200154 [doi]
AB  - To overcome the critical barriers in gene delivery, a series of reducible 
      polycations (RPCs) based on low molecular weight (LMW) peptides, i.e. PolyHK6 H, 
      PolyHK6 H-mPEG1 , PolyHK6 H-mPEG2 , and PolyHK6 H-mPEG3 , with different 
      poly(ethylene glycol) (PEG) contents, are synthesized and evaluated as nonviral 
      gene vectors. All the RPCs exhibit lower cytotoxicity compared with 25 kDa 
      polyethyleneimine (PEI) and PEGylated PEI (PEI-mPEG: PEI-mPEG1 , PEI-mPEG2 , and 
      PEI-mPEG3 ). PolyHK6 H-mPEG1 and PolyHK6 H-mPEG2 can bind and condense plasmid 
      deoxyribonucleic acid (pDNA) efficiently with a particle size of about 200 nm. 
      Moreover, they display much higher transfection efficiency than that of 25 kDa 
      PEI especially in serum-supplemented medium. Moreover, PolyHK6 H-mPEG1 has equal 
      transfection efficiency with PEI-mPEG1 which is optimal in the PEI-mPEG, but 
      PolyHK6 H-mPEG1 exhibits significantly lower cytotoxicity than PEI-mPEG1 . This 
      is attributed to the fact that inter-peptide disulfide bonds can increase the 
      stability of RPCs/pDNA complexes in extracellular environment and thereafter 
      cleave in cytoplasm to facilitate the release of pDNA in intracellular 
      environment. The PEGylated RPCs demonstrate here improved intracellular gene 
      transfer performance and will have great potential applications in vivo.
CI  - Copyright (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Yang, Juan
AU  - Yang J
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education & Department of 
      Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Wang, Hui-Yuan
AU  - Wang HY
FAU - Yi, Wen-Jie
AU  - Yi WJ
FAU - Gong, Yu-Hui
AU  - Gong YH
FAU - Zhou, Xiang
AU  - Zhou X
FAU - Zhuo, Ren-Xi
AU  - Zhuo RX
FAU - Zhang, Xian-Zheng
AU  - Zhang XZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121004
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Drug Carriers)
RN  - 0 (Peptides)
RN  - 0 (Polyamines)
RN  - 0 (Polyelectrolytes)
RN  - 0 (polycations)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - DNA/chemistry/genetics/*pharmacokinetics
MH  - Drug Carriers/chemistry
MH  - Drug Stability
MH  - Electrophoretic Mobility Shift Assay
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors/*chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - Particle Size
MH  - Peptides/*chemistry
MH  - Polyamines/*chemistry
MH  - Polyelectrolytes
MH  - Polyethylene Glycols/*chemistry
EDAT- 2012/11/28 06:00
MHDA- 2013/06/26 06:00
CRDT- 2012/11/28 06:00
PHST- 2012/05/10 00:00 [received]
PHST- 2012/08/28 00:00 [revised]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - 10.1002/adhm.201200154 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2013 Mar;2(3):481-9. doi: 10.1002/adhm.201200154. Epub 2012 
      Oct 4.