PMID- 23185205
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121128
LR  - 20220408
IS  - 1896-9151 (Electronic)
IS  - 1734-1922 (Print)
IS  - 1734-1922 (Linking)
VI  - 8
IP  - 5
DP  - 2012 Nov 9
TI  - Dexamethasone and vitamin B(12) synergistically promote peripheral nerve 
      regeneration in rats by upregulating the expression of brain-derived neurotrophic 
      factor.
PG  - 924-30
LID - 10.5114/aoms.2012.31623 [doi]
AB  - INTRODUCTION: Dexamethasone and vitamin B(12) are currently used in the clinic to 
      treat peripheral nerve damage but their mechanisms of action remain incompletely 
      understood. In this study we hypothesized that dexamethasone and vitamin B(12) 
      promote the production of endogenous neurotrophic factors, thereby enhancing 
      peripheral nerve repair. MATERIAL AND METHODS: Ninety-six adult male Wistar rats 
      were employed to establish a sciatic nerve injury model. They were then randomly 
      divided into 4 groups to be subjected to different treatment: saline (group A), 
      dexamethasone (group B), vitamin B(12) (group C), and dexamethasone combined with 
      vitamin B(12) (group D). The walking behavior of rats was evaluated by footprint 
      analysis, and the nerve regeneration was assessed by electrophysiological 
      analysis and ultrastructural examination. The expression of brain-derived 
      neurotrophic factor (BDNF), glial cell-derived neurotrophic factor, NT-3 and IL-6 
      in the injured sciatic nerves was detected by immunohistochemical and RT-PCR 
      analysis. RESULTS: Dexamethasone and vitamin B(12) promoted the regeneration of 
      myelinated nerve fibers and the proliferation of Schwann cells. Furthermore, 
      dexamethasone and vitamin B(12) promoted the recovery of sciatic functional index 
      and sensory nerve conduction velocity, and upregulated BDNF expression in the 
      injured sciatic nerves. CONCLUSIONS: Dexamethasone and vitamin B(12) promote 
      peripheral nerve repair in a rat model of sciatic nerve injury through the 
      upregulation of BDNF expression. These findings provide new insight into the 
      neurotrophic effects of dexamethasone and vitamin B(12) and support the 
      application of these agents in clinical treatment of peripheral nerve injury.
FAU - Sun, Hongzhi
AU  - Sun H
AD  - Department of General Surgery, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou, China.
FAU - Yang, Tao
AU  - Yang T
FAU - Li, Qing
AU  - Li Q
FAU - Zhu, Zhitu
AU  - Zhu Z
FAU - Wang, Lei
AU  - Wang L
FAU - Bai, Guang
AU  - Bai G
FAU - Li, Dongsheng
AU  - Li D
FAU - Li, Qiang
AU  - Li Q
FAU - Wang, Wei
AU  - Wang W
LA  - eng
PT  - Journal Article
DEP - 20121107
PL  - Poland
TA  - Arch Med Sci
JT  - Archives of medical science : AMS
JID - 101258257
PMC - PMC3506245
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - dexamethasone
OT  - peripheral nerve repair
OT  - sciatic nerve
OT  - vitamin B12
EDAT- 2012/11/28 06:00
MHDA- 2012/11/28 06:01
PMCR- 2012/11/09
CRDT- 2012/11/28 06:00
PHST- 2012/02/09 00:00 [received]
PHST- 2012/03/11 00:00 [revised]
PHST- 2012/04/10 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2012/11/28 06:01 [medline]
PHST- 2012/11/09 00:00 [pmc-release]
AID - 19716 [pii]
AID - 10.5114/aoms.2012.31623 [doi]
PST - ppublish
SO  - Arch Med Sci. 2012 Nov 9;8(5):924-30. doi: 10.5114/aoms.2012.31623. Epub 2012 Nov 
      7.