PMID- 23185681
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121128
LR  - 20220309
IS  - 2036-2579 (Electronic)
IS  - 1826-1868 (Print)
IS  - 1826-1868 (Linking)
VI  - 7
IP  - 2
DP  - 2012 Jun 5
TI  - Development of bioartificial myocardium by electrostimulation of 3D collagen 
      scaffolds seeded with stem cells.
PG  - e14
LID - 10.4081/hi.2012.e14 [doi]
LID - e14
AB  - Electrostimulation (ES) can be defined as a safe physical method to induce stem 
      cell differentiation. The aim of this study is to evaluate the effectiveness of 
      ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in 
      terms of proliferation and differentiation into cardiomyocytes. BMSCs were 
      isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring 
      25 x 25 x 6 mm. Bipolar in vitro ES was performed during 21 days. Electrical 
      impedance and cell proliferation were measured. Expression of cardiac markers was 
      assessed by immunocytochemistry. Viscoelasticity of collagen matrix was 
      evaluated. Electrical impedance assessments showed a low resistance of 234+/-41 
      Ohms which indicates good electrical conductivity of collagen matrix. Cell 
      proliferation at 570 nm as significantly increased in ES groups after seven day 
      (ES 0.129+/-0.03 vs non-stimulated control matrix 0.06+/-0.01, P=0.002) and after 21 
      days, (ES 0.22+/-0.04 vs control 0.13+/-0.01, P=0.01). Immunocytoche mistry of BMSCs 
      after 21 days ES showed positive staining of cardiac markers, troponin I, 
      connexin 43, sarcomeric alpha-actinin, slow myosin, fast myosin and desmin. 
      Staining for BMSCs marker CD29 after 21 days was negative. Electrostimulation of 
      cell-seeded collagen matrix changed stem cell morphology and biochemical 
      characteristics, increasing the expression of cardiac markers. Thus, MSC-derived 
      differentiated cells by electrostimulation grafted in biological scaffolds might 
      result in a convenient tissue engineering source for myocardial diseases.
FAU - Haneef, Kanwal
AU  - Haneef K
AD  - University Paris Descartes, Pompidou Hospital, Laboratory of Biosurgical Research 
      (LRB), Paris;
FAU - Lila, Nermine
AU  - Lila N
FAU - Benadda, Samira
AU  - Benadda S
FAU - Legrand, Fabien
AU  - Legrand F
FAU - Carpentier, Alain
AU  - Carpentier A
FAU - Chachques, Juan C
AU  - Chachques JC
LA  - eng
PT  - Journal Article
DEP - 20120918
PL  - England
TA  - Heart Int
JT  - Heart international
JID - 101541778
PMC - PMC3504306
OTO - NOTNLM
OT  - bioartificial myocardium.
OT  - cell conditioning
OT  - collagen scaffold
OT  - electrostimulation
OT  - heart failure
OT  - myocardial infarct
OT  - stem cells
COIS- Conflict of interests: the authors report no potential conflict of interests.
EDAT- 2012/11/28 06:00
MHDA- 2012/11/28 06:01
PMCR- 2012/09/18
CRDT- 2012/11/28 06:00
PHST- 2012/04/20 00:00 [received]
PHST- 2012/08/02 00:00 [revised]
PHST- 2012/08/06 00:00 [accepted]
PHST- 2012/11/28 06:00 [entrez]
PHST- 2012/11/28 06:00 [pubmed]
PHST- 2012/11/28 06:01 [medline]
PHST- 2012/09/18 00:00 [pmc-release]
AID - hi.2012.e14 [pii]
AID - 10.4081/hi.2012.e14 [doi]
PST - ppublish
SO  - Heart Int. 2012 Jun 5;7(2):e14. doi: 10.4081/hi.2012.e14. Epub 2012 Sep 18.