PMID- 23185968
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20141120
IS  - 1741-4520 (Electronic)
IS  - 0914-3505 (Linking)
VI  - 53
IP  - 1
DP  - 2013 Mar
TI  - In vitro evaluation of gene expression changes for gonadotropin-releasing hormone 
      1, brain-derived neurotrophic factor and neurotrophic tyrosine kinase, receptor, 
      type 2, in response to bisphenol A treatment.
PG  - 42-5
AB  - We evaluated the effects of bisphenol A (BPA) on embryonic mouse hypothalamic 
      cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) 
      indicated that gonadotropin-releasing hormone 1 (Gnrh1) expression in 0.02-20 muM 
      BPA-treated cells did not differ from that in control cells but decreased 
      significantly in 200 muMBPAtreated cells. The mRNA level for brain-derived 
      neurotrophic factor (Bdnf), which participates in GNRH1 secretory system 
      development, decreased significantly in 200 muM BPA-treated cells, but that for 
      neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), did not change. This 
      indicates that Gnrh1 gene expression in mice fetuses is not affected by exposure 
      to <20 muM BPA and that the adverse effects of BPA on the BDNF-NTRK2 neurotrophin 
      system are induced by decrease in the mRNA level of the ligand, not of its 
      receptor.
FAU - Warita, Katsuhiko
AU  - Warita K
AD  - Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, 
      Kita-gun, Kagawa, Japan.
FAU - Mitsuhashi, Tomoko
AU  - Mitsuhashi T
FAU - Ohta, Ken-ichi
AU  - Ohta K
FAU - Suzuki, Shingo
AU  - Suzuki S
FAU - Hoshi, Nobuhiko
AU  - Hoshi N
FAU - Miki, Takanori
AU  - Miki T
FAU - Takeuchi, Yoshiki
AU  - Takeuchi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Congenit Anom (Kyoto)
JT  - Congenital anomalies
JID - 9306292
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Phenols)
RN  - 0 (beta Catenin)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Animals
MH  - Benzhydryl Compounds/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Gene Expression Regulation
MH  - Gonadotropin-Releasing Hormone/*genetics
MH  - In Vitro Techniques
MH  - Mice
MH  - Phenols/*pharmacology
MH  - Pregnancy
MH  - Receptor, trkB/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - beta Catenin/physiology
EDAT- 2012/11/29 06:00
MHDA- 2013/08/30 06:00
CRDT- 2012/11/29 06:00
PHST- 2012/06/19 00:00 [accepted]
PHST- 2012/11/29 06:00 [entrez]
PHST- 2012/11/29 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
AID - 10.1111/j.1741-4520.2012.00381.x [doi]
PST - ppublish
SO  - Congenit Anom (Kyoto). 2013 Mar;53(1):42-5. doi: 
      10.1111/j.1741-4520.2012.00381.x.