PMID- 23187120
OWN - NLM
STAT- MEDLINE
DCOM- 20130211
LR  - 20131121
IS  - 1538-2990 (Electronic)
IS  - 0002-9629 (Linking)
VI  - 344
IP  - 6
DP  - 2012 Dec
TI  - Spinal MCP-1 contributes to the development of morphine antinociceptive tolerance 
      in rats.
PG  - 473-9
LID - 10.1097/MAJ.0b013e31826a82ce [doi]
AB  - BACKGROUND: The chemokine monocyte chemoattractant protein-1 (MCP-1) has been 
      shown to contribute to neuropathic pain. However, whether MCP-1 is involved in 
      the development of morphine antinociceptive tolerance is incompletely understood. 
      METHODS: Morphine antinociceptive tolerance was induced by intrathecal 
      administration of 15 mug of morphine daily for 7 days. Immunohistochemistry was 
      used to test the changes in the morphology of spinal MCP-1 immunoreactivity and 
      OX-42-IR. The role of MCP-1 in morphine antinociceptive tolerance is explored by 
      hot-water tail-flick test. RESULTS: Our findings showed that intrathecal chronic 
      morphine exposure obviously increased MCP-1 immunoreactivity in the spinal cord. 
      Moreover, the increased MCP-1 immunoreactivity was observed mainly in the spinal 
      neurons. Intrathecal injections of MCP-1-neutralizing antibody significantly 
      reduced the development of morphine antinociceptive tolerance, suggesting that 
      spinal neuronal MCP-1 contributes to tolerance to morphine antinociception. 
      Treatment with MCP-1-neutralizing antibody also reduced the spinal microglial 
      activation induced by chronic morphine treatment. CONCLUSIONS: This study 
      revealed for the first time that spinal neuronal MCP-1 is a key mediator of the 
      spinal microglial activation and that spinal MCP-1 is involved in morphine 
      antinociceptive tolerance. Inhibition of MCP-1 may provide a new therapy for 
      morphine tolerance management.
FAU - Zhao, Chun-mei
AU  - Zhao CM
AD  - Department of Physiology, Zhongshan Medical College, Sun Yat-sen University, 
      Guangzhou, China.
FAU - Guo, Rui-xian
AU  - Guo RX
FAU - Hu, Fen
AU  - Hu F
FAU - Meng, Jin-lan
AU  - Meng JL
FAU - Mo, Li-qiu
AU  - Mo LQ
FAU - Chen, Pei-xi
AU  - Chen PX
FAU - Liao, Xin-xue
AU  - Liao XX
FAU - Cui, Yu
AU  - Cui Y
FAU - Feng, Jian-qiang
AU  - Feng JQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Med Sci
JT  - The American journal of the medical sciences
JID - 0370506
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Analgesics, Opioid/*administration & dosage
MH  - Animals
MH  - Antibodies, Neutralizing/administration & dosage
MH  - Chemokine CCL2/antagonists & inhibitors/immunology/*physiology
MH  - Drug Tolerance/*physiology
MH  - Immunohistochemistry
MH  - Male
MH  - Microglia/drug effects/physiology
MH  - Morphine/*administration & dosage
MH  - Nociception/drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/drug effects/*physiology
MH  - Up-Regulation/drug effects
EDAT- 2012/11/29 06:00
MHDA- 2013/02/12 06:00
CRDT- 2012/11/29 06:00
PHST- 2012/11/29 06:00 [entrez]
PHST- 2012/11/29 06:00 [pubmed]
PHST- 2013/02/12 06:00 [medline]
AID - S0002-9629(15)30788-6 [pii]
AID - 10.1097/MAJ.0b013e31826a82ce [doi]
PST - ppublish
SO  - Am J Med Sci. 2012 Dec;344(6):473-9. doi: 10.1097/MAJ.0b013e31826a82ce.