PMID- 23188065
OWN - NLM
STAT- MEDLINE
DCOM- 20140110
LR  - 20171116
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 138
IP  - 2
DP  - 2013 Jan 21
TI  - Novel colorimetric molecular switch based on copper(I)-catalyzed azide-alkyne 
      cycloaddition reaction and its application for flumioxazin detection.
PG  - 688-92
LID - 10.1039/c2an36023c [doi]
AB  - A novel colorimetric switch based on the copper(I)-catalyzed azide-alkyne 
      cycloaddition (CuAAC) reaction has been developed. G-quadruplex-hemin DNAzyme 
      catalyzes the oxidation of 2,2'-azinobis(3-ethylbenzothiozoline)-6-sulfonic acid 
      (ABTS) to form ABTS (+), the UV absorbance of the solution increased greatly and 
      the color of the solution changed to dark green. However, in the presence of an 
      azide complex, the absorbance signal decreased and the solution became light 
      green since the catalytic ability of the hemin was inhibited by the azide groups. 
      However, once propargylamine has been added into the above reaction system, which 
      would react with azide groups through the CuAAC reaction, the solution becomes 
      dark green again and the absorption intensity of the system is also increased. 
      The proposed switch allows a good reversibility and can be identified clearly by 
      the naked eye. In addition, the method has been applied to detect some 
      pesticides, which have alkynyl groups (flumioxazin), with high sensitivity and 
      selectivity, where the UV absorbance has a direct linear relationship with the 
      logarithm of flumioxazin concentrations in the range of 0.14-14 nM, and the limit 
      of detection was 0.056 nM (S/N = 3), which can meet the requirement of the 
      maximum residue limits (MRLs) of United States of America (56 nM).
FAU - Xie, Lidan
AU  - Xie L
AD  - Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, 
      Fuzhou University, Fuzhou, Fujian 350002, China.
FAU - Zheng, Hanye
AU  - Zheng H
FAU - Ye, Wenmei
AU  - Ye W
FAU - Qiu, Suyan
AU  - Qiu S
FAU - Lin, Zhenyu
AU  - Lin Z
FAU - Guo, Longhua
AU  - Guo L
FAU - Qiu, Bin
AU  - Qiu B
FAU - Chen, Guonan
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Alkynes)
RN  - 0 (Azides)
RN  - 0 (Benzoxazines)
RN  - 0 (Pesticides)
RN  - 0 (Phthalimides)
RN  - 789U1901C5 (Copper)
RN  - L0PX7OGI22 (flumioxazin)
SB  - IM
MH  - Alkynes/chemistry
MH  - Azides/chemistry
MH  - Benzoxazines/*analysis
MH  - *Biosensing Techniques
MH  - Colorimetry/*methods
MH  - Copper/chemistry
MH  - *Cycloaddition Reaction
MH  - Pesticides/*analysis
MH  - Phthalimides/*analysis
EDAT- 2012/11/29 06:00
MHDA- 2014/01/11 06:00
CRDT- 2012/11/29 06:00
PHST- 2012/11/29 06:00 [entrez]
PHST- 2012/11/29 06:00 [pubmed]
PHST- 2014/01/11 06:00 [medline]
AID - 10.1039/c2an36023c [doi]
PST - ppublish
SO  - Analyst. 2013 Jan 21;138(2):688-92. doi: 10.1039/c2an36023c.