PMID- 23189042
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121129
LR  - 20211021
IS  - 1662-5110 (Electronic)
IS  - 1662-5110 (Linking)
VI  - 6
DP  - 2012
TI  - Molecular mechanism of parallel fiber-Purkinje cell synapse formation.
PG  - 90
LID - 10.3389/fncir.2012.00090 [doi]
LID - 90
AB  - The cerebellum receives two excitatory afferents, the climbing fiber (CF) and the 
      mossy fiber-parallel fiber (PF) pathway, both converging onto Purkinje cells 
      (PCs) that are the sole neurons sending outputs from the cerebellar cortex. 
      Glutamate receptor delta2 (GluRdelta2) is expressed selectively in cerebellar PCs and 
      localized exclusively at the PF-PC synapses. We found that a significant number 
      of PC spines lack synaptic contacts with PF terminals and some of residual PF-PC 
      synapses show mismatching between pre- and postsynaptic specializations in 
      conventional and conditional GluRdelta2 knockout mice. Studies with mutant mice 
      revealed that in addition to PF-PC synapse formation, GluRdelta2 is essential for 
      synaptic plasticity, motor learning, and the restriction of CF territory. GluRdelta2 
      regulates synapse formation through the amino-terminal domain, while the control 
      of synaptic plasticity, motor learning, and CF territory is mediated through the 
      carboxyl-terminal domain. Thus, GluRdelta2 is the molecule that bridges synapse 
      formation and motor learning. We found that the trans-synaptic interaction of 
      postsynaptic GluRdelta2 and presynaptic neurexins (NRXNs) through cerebellin 1 
      (Cbln1) mediates PF-PC synapse formation. The synaptogenic triad is composed of 
      one molecule of tetrameric GluRdelta2, two molecules of hexameric Cbln1 and four 
      molecules of monomeric NRXN. Thus, GluRdelta2 triggers synapse formation by 
      clustering four NRXNs. These findings provide a molecular insight into the 
      mechanism of synapse formation in the brain.
FAU - Mishina, Masayoshi
AU  - Mishina M
AD  - Brain Science Laboratory, The Research Organization of Science and Technology, 
      Ritsumeikan University Shiga, Japan ; Molecular Neurobiology and Pharmacology, 
      Graduate School of Medicine, The University of Tokyo Tokyo, Japan.
FAU - Uemura, Takeshi
AU  - Uemura T
FAU - Yasumura, Misato
AU  - Yasumura M
FAU - Yoshida, Tomoyuki
AU  - Yoshida T
LA  - eng
PT  - Journal Article
DEP - 20121123
PL  - Switzerland
TA  - Front Neural Circuits
JT  - Frontiers in neural circuits
JID - 101477940
PMC - PMC3505014
OTO - NOTNLM
OT  - Purkinje cell
OT  - glutamate receptor delta2
OT  - motor learning
OT  - neurexin
OT  - parallel fiber
OT  - synapse formation
EDAT- 2012/11/29 06:00
MHDA- 2012/11/29 06:01
PMCR- 2012/01/01
CRDT- 2012/11/29 06:00
PHST- 2012/08/30 00:00 [received]
PHST- 2012/11/02 00:00 [accepted]
PHST- 2012/11/29 06:00 [entrez]
PHST- 2012/11/29 06:00 [pubmed]
PHST- 2012/11/29 06:01 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 10.3389/fncir.2012.00090 [doi]
PST - epublish
SO  - Front Neural Circuits. 2012 Nov 23;6:90. doi: 10.3389/fncir.2012.00090. 
      eCollection 2012.