PMID- 23189660
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 7
DP  - 2012 Jul
TI  - [Analysis of six phenolic environmental estrogens in bullfrog blood by using 
      dispersive solid-phase extraction and ultrafast liquid chromatography-tandem mass 
      spectrometry].
PG  - 665-71
AB  - A rapid, sensitive and accurate method for the simultaneous determination of six 
      phenolic environmental estrogens, i. e., bisphenol A (BPA), diethylstilbestrol 
      (DES), dienestrol( DE), hexestrol (HEX), 4-( tert-octyl)-phenol (4-tOP) and 
      4-nonylphenol (4-NP) in bullfrog blood by dispersive solid-phase 
      extraction-ultrafast liquid chromatography-tandem mass spectrometry 
      (dSPE-UFLC-MS/MS) was established. After protein precipitation, bullfrog blood 
      samples were cleaned-up by dSPE method with ethylenediamine-functionalized Fe3O4, 
      magnetic polymers (EDA-MPs) as adsorbent. The effects of precipitation solvents, 
      adsorption time and the amount of EDA-MPs used on the recoveries of six phenolic 
      environmental estrogens were investigated in detail. Chromatographic separation 
      was performed on a Shim-pack XR-ODS II analytical column (100 mm x 2.0 mm, 2.2 
      microm). The mass spectrometer was operated by using electrospray ion (ESI) 
      source in the multiple reaction monitoring (MRM) mode. The results showed that 
      the linearities were in the range of 0.5-100.0 microg/L with correlation 
      coefficients (r2) not less than 0.999 6 for all the six phenolic environmental 
      estrogens. The lim- its of quantification (LOQs) (S/N > 10) in bullfrog blood 
      samples were between 0. 075 microg/L and 0.40 microg/L. The recoveries were 
      between 95.0% and 110.0% at three spiked levels. The precision values expressed 
      as relative standard deviations (RSDs) were in the range of 0.6%-6.3%. The 
      developed method can be applied to the routine analysis of the six phenolic 
      environmental estrogens in bullfrog blood samples.
FAU - Zhao, Yonggang
AU  - Zhao Y
AD  - Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for 
      Disease Control and Prevention, Ningbo 315010. China.
FAU - Chen, Xiaohong
AU  - Chen X
FAU - Yao, Shanshan
AU  - Yao S
FAU - Li, Xiaoping
AU  - Li X
FAU - Jin, Micong
AU  - Jin M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 0 (Phenols)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - MLT3645I99 (bisphenol A)
RN  - RRW32X4U1F (Dienestrol)
SB  - IM
MH  - Animals
MH  - Benzhydryl Compounds/blood
MH  - Chromatography, Liquid/*methods
MH  - Dienestrol/blood
MH  - Diethylstilbestrol/blood
MH  - Environmental Pollutants/*blood
MH  - Estrogens, Non-Steroidal/*blood
MH  - Phenols/*blood
MH  - Rana catesbeiana/blood
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/11/30 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/11/30 06:00
PHST- 2012/11/30 06:00 [entrez]
PHST- 2012/11/30 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.02028 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jul;30(7):665-71. doi: 10.3724/sp.j.1123.2012.02028.