PMID- 23189661
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 7
DP  - 2012 Jul
TI  - [Determination of rhodamine B in spices by solid phase extraction-high 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 672-6
AB  - Rhodamine B (RB), as an unlawful colour, is forbidden to add into foods by 
      Chinese government. A solid phase extraction-high performance liquid 
      chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for the 
      determination of RB in spices has been developed. The sample was extracted by 
      acetonitrile and then centrifugated, purified and enriched with a strong positive 
      ion exchange SPE column (Bond Elut Plexa PCX SPE column) after adding 10 mL 1% 
      trichloroacetic acid solution. The HPLC separation was performed on a Pursuit C18 
      column (100 mm x 2.0 mm, 3 microm) by gradient elution with 0.1% (v/v) formic 
      acid solution and methanol as the mobile phase. The analyte was detected by 
      electrospray ionization in positive ion mode-MS/MS in multiple reaction 
      monitoring (MRM) mode. The good linearity (R2 > 0.99) was obtained over the range 
      of 0.6-6 microg/L. The limit of quantification (LOQ) for RB was 1.2 microg/kg. 
      The average recoveries were ranged from 80% to 121% at the spiked levels of 
      1.197, 2.992 and 5.985 microg/L, and the relative standard deviations (RSDs) were 
      not more than 15%. The conditions of mobile phase elution gradients, extraction 
      solvents, and SPE columns were optimized. This method is highly selective and has 
      weak matrix effect for qualitative and quantitative analyses of RB in spices.
FAU - Yin, Feng
AU  - Yin F
AD  - State Non-Staple Food Quality Supervision and Test Center, Beijing 100070, China. 
      fifayinfeng@yahoo.com.cn
FAU - Ding, Zhaowei
AU  - Ding Z
FAU - Yang, Zhijian
AU  - Yang Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Rhodamines)
RN  - K7G5SCF8IL (rhodamine B)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fluorescent Dyes/analysis
MH  - Food Contamination/analysis
MH  - Rhodamines/*analysis
MH  - Solid Phase Extraction/*methods
MH  - Spices/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/11/30 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/11/30 06:00
PHST- 2012/11/30 06:00 [entrez]
PHST- 2012/11/30 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.02016 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jul;30(7):672-6. doi: 10.3724/sp.j.1123.2012.02016.