PMID- 23189666
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 7
DP  - 2012 Jul
TI  - [Determination of cefuroxime in liver-injured rat plasma by ultra fast liquid 
      chromatography-tandem mass spectrometry via acidified protein precipitation].
PG  - 705-10
AB  - In order to investigate the pharmacokinetic profiles of cefuroxime lysine, a new 
      second generation cephalosporins, in liver-injured rat model, an ultra fast 
      liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the 
      determination of cefuroxime in liver-injured rat plasma was developed and 
      validated. The plasma sample was pretreated by protein precipitation with 
      acidified acetonitrile. The analytes were separated on a Shim-pack XR-ODS column 
      (75 mm x 3.0 mm, 2.2 microm) with acetonitrile-0. 1% formic acid aqueous solution 
      (40:60, v/v) as the mobile phase at a flow rate of 400 microL/min. The mass 
      spectrometer was operated in multiple reaction monitoring (MRM) mode with a 
      negative electrospray ionization (ESI) interface. The precursor to product ion 
      transitions of m/z 423.2 --> 206.8 and m/z 454.1 --> 238.4 were selected to 
      determine cefuroxime and cefotaxime (internal standard, IS), respectively. The 
      linearities ranged from 0.01 to 1 mg/L and 1 to 400 mg/L (r > 0.99), and the 
      limit of quantification of cefuroxime was 0.01 mg/L. The relative standard 
      deviations (RSDs) of intra- and inter-day precisions were both less than 11.5%, 
      and the accuracy (relative error) was between -7.1% and 2.2%. The mean extraction 
      recovery was more than 83.5%. The total run time was 3.0 min per sample. The 
      method is simple and fast for the preliminary pharmacokinetic study of cefuroxime 
      lysine in liver-injured rats.
FAU - Zhao, Longshan
AU  - Zhao L
AD  - School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Li, Qing
AU  - Li Q
FAU - Yang, Wei
AU  - Yang W
FAU - He, Bosai
AU  - He B
FAU - Wei, Binbin
AU  - Wei B
FAU - Liu, Ran
AU  - Liu R
FAU - Liu, Jingjing
AU  - Liu J
FAU - Chen, Xiaohui
AU  - Chen X
FAU - Bi, Kaishun
AU  - Bi K
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Proteins)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - O1R9FJ93ED (Cefuroxime)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride
MH  - Cefuroxime/*blood/pharmacokinetics
MH  - Chemical Precipitation
MH  - Chemical and Drug Induced Liver Injury/*blood/etiology
MH  - Chromatography, Liquid/*methods
MH  - Proteins/chemistry
MH  - Rats
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/11/30 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/11/30 06:00
PHST- 2012/11/30 06:00 [entrez]
PHST- 2012/11/30 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.03001 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jul;30(7):705-10. doi: 10.3724/sp.j.1123.2012.03001.