PMID- 23189667
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 7
DP  - 2012 Jul
TI  - [Fast determination of adenosine and cordycepin in Cordyceps and its deserted 
      solid medium].
PG  - 711-5
AB  - A fast analytical method for adenosine and cordycepin in Cordyceps and its 
      deserted solid medium was developed by using a normal-phase cyan-group 
      chromatographic column. The sample was extracted for 1.5 min using a 
      microwave-assisted extraction system. The extraction was repeated twice. The 
      analysis of adenosine and cordycepin was performed on an Eclipse XDB-CN column. 
      The mobile phase was composed of methanol and water with a ratio of 7:93 (v/v) 
      for isocratic elution. The detection wavelength was 260 nm. The composition and 
      pH value of the mobile phase were investigated. The results showed that adenosine 
      and cordycepin could be completely separated without matrix interference in 4.5 
      min. The linearity of the method was good with a linear correlation coefficient 
      (r2) of 0. 999 8 for adenosine and 0.999 5 for cordycepin. The limits of 
      quantification (LOQs, S/N = 10) of adenosine and cordycepin were 0.21 and 0.083 
      mg/L, respectively. The relative standard deviations (RSDs) of peak areas of six 
      replicate injections were less than 2% both for intra-day and inter-day analysis. 
      The average recoveries of adenosine and cordycepin ranged from 93.8% to 102.9% 
      with the RSD not more than 3.62% (n = 5). The developed method is simple, fast, 
      accurate and low-cost, and it can be used in the fast detection of adenosine and 
      cordycepin in Cordyceps, carpohole, the deserted solid medium and its 
      praeparatum.
FAU - Li, Chen
AU  - Li C
AD  - Faculty of Chemical Engineering and Light Industry, Guangdong University of 
      Technology, Guangzhou 510006. China. simmonlee@163.com
FAU - Yan, Aiguo
AU  - Yan A
FAU - Cai, Chunyan
AU  - Cai C
FAU - Liu, Zhiping
AU  - Liu Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Culture Media)
RN  - 0 (Deoxyadenosines)
RN  - GZ8VF4M2J8 (cordycepin)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*analysis
MH  - Chromatography, High Pressure Liquid/*instrumentation/*methods
MH  - Cordyceps/*chemistry/growth & development
MH  - Culture Media/chemistry
MH  - Deoxyadenosines/*analysis
EDAT- 2012/11/30 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/11/30 06:00
PHST- 2012/11/30 06:00 [entrez]
PHST- 2012/11/30 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.02037 [doi]
PST - ppublish
SO  - Se Pu. 2012 Jul;30(7):711-5. doi: 10.3724/sp.j.1123.2012.02037.