PMID- 23190578
OWN - NLM
STAT- MEDLINE
DCOM- 20140127
LR  - 20130716
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 35
IP  - 4
DP  - 2013 Aug
TI  - Interphase-FISH screening for eight common rearrangements in pediatric B-cell 
      precursor acute lymphoblastic leukemia.
PG  - 406-15
LID - 10.1111/ijlh.12031 [doi]
AB  - INTRODUCTION: This is the first pilot study to screen multiple common genetic 
      aberrations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). METHODS: 
      Thirty-two children with BCP-ALL were investigated for chromosomal rearrangements 
      using interphase fluorescence in situ hybridization (FISH). Eight common 
      translocations and rearrangements, including ETV6-RUNX1, TCF3-PBX1, BCR-ABL1, 
      ETV6, TCF3, MLL, IGH@, and PAX5, were tested for using dual-color DNA probes. 
      RESULTS: ETV6-RUNX1 was the most frequent translocation detected in 11 children 
      (34.4%). Two patients with BCR-ABL1 (6.3%) and one with TCF3-PBX1 (3.1%) 
      translocations were also observed. Using break-apart probes, 11 children (34.4%) 
      had a positive FISH result for ETV6, two patients for IGH@ (6.3%), one patient 
      for MLL (3.1%), and one patient for PAX5 rearrangements (3.1%). All patients with 
      the ETV6-RUNX1 fusion were also identified by split signals for ETV6. Other 
      abnormalities, including extra copies and deletion of genes, were observed within 
      the range of 3.1-34.4%. Cytogenetics analysis showed a single case each of 
      BCR-ABL1 fusion, MLL, and IGH@ rearrangements (3.1% each). ETV6-RUNX1 fusion and 
      ETV6 split-apart rearrangements were not visible by cytogenetics. Likewise, one 
      each of cases with TCF3-PBX1 fusion and with PAX5 split signal seen by FISH was 
      not visible by cytogenetics. CONCLUSION: By using 8 FISH probes in conjunction 
      cytogenetics for the detection of common aberrations, interphase FISH enhanced 
      the detection of chromosomal rearrangements in children with BCP-ALL.
CI  - (c) 2012 John Wiley & Sons Ltd.
FAU - Hutspardol, S
AU  - Hutspardol S
AD  - Department of Pediatrics, Srinakharinwirot University, Nakorn Nayok, Thailand.
FAU - Pakakasama, S
AU  - Pakakasama S
FAU - Kanta, K
AU  - Kanta K
FAU - Nuntakarn, L
AU  - Nuntakarn L
FAU - Anurathapan, U
AU  - Anurathapan U
FAU - Sirachainan, N
AU  - Sirachainan N
FAU - Songdej, D
AU  - Songdej D
FAU - Sawangpanich, R
AU  - Sawangpanich R
FAU - Tiyasirichokchai, R
AU  - Tiyasirichokchai R
FAU - Rerkamnuaychoke, B
AU  - Rerkamnuaychoke B
FAU - Hongeng, S
AU  - Hongeng S
LA  - eng
PT  - Journal Article
DEP - 20121128
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - B-Lymphocytes/metabolism/*pathology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*statistics & numerical data
MH  - Infant
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Male
MH  - Oncogene Proteins, Fusion/*genetics/metabolism
MH  - Pilot Projects
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis/*genetics/pathology
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - ALL
OT  - B-cells
OT  - FISH
EDAT- 2012/11/30 06:00
MHDA- 2014/01/28 06:00
CRDT- 2012/11/30 06:00
PHST- 2011/12/27 00:00 [received]
PHST- 2012/09/28 00:00 [accepted]
PHST- 2012/11/30 06:00 [entrez]
PHST- 2012/11/30 06:00 [pubmed]
PHST- 2014/01/28 06:00 [medline]
AID - 10.1111/ijlh.12031 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2013 Aug;35(4):406-15. doi: 10.1111/ijlh.12031. Epub 2012 Nov 
      28.