PMID- 23196073
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121203
LR  - 20200930
IS  - 0176-1617 (Print)
IS  - 0176-1617 (Linking)
VI  - 118
IP  - 4
DP  - 1985 Mar
TI  - Kinetics and Specificity of ATP-dependent Proton Translocation Measured with 
      Acridine Orange in Microsomal Fractions from Green Suspension Cells of 
      Chenopodium rubrum L.
PG  - 309-25
LID - S0176-1617(85)80190-5 [pii]
LID - 10.1016/S0176-1617(85)80190-5 [doi]
AB  - From photoautotrophic cell suspension cultures of Chenopodium rubrum L. 
      microsomal fractions were prepared by differential and isopycnic sucrose gradient 
      centrifugation. ATP-generated proton-accumulation was studied on a microsomal 
      fraction (MF) of proton-translocating vesicles with a density of 1.11 to 1.12 
      gem(-3). This activity did not overlap with mitochondrial or chloroplast markers. 
      MF is presumably of tonoplast origin. The assay was based upon pH-dependent 
      acridine orange (AO) absorption shifts at 490 nm, Delta(490), due to AO-accumulation 
      and polymerization within the vesicles, and was calibrated using pK and K(D) for 
      AO polymers given by Zanker (Z. physikal. Chemie 199, 225, 1952). Addition of 1 
      mM ATP to MF yields a transmembrane DeltapH of about 2 units within 10 min (kinetics 
      with a single time constant, tau=400s) and collapses after addition of the 
      protonophor CCCP (tau=22s). The proton pumping activity is insensitive to vanadate 
      and highly specific for ATP compared with GTP, UTP, CTP, and ITP, and 
      half-saturates at 0.38 to 0.46 mM ATP, indicated by both initial rate (1 min) and 
      steady state value of Delta(490) (10 min). Proton pumping into and proton leakage 
      from the vesicles was cast into a feedback-scheme showing that the pump is 
      working at a constant rate, that is, independently of the size of the created 
      DeltapH.
CI  - Copyright (c) 1985 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. 
      All rights reserved.
FAU - Gogarten-Boekels, M
AU  - Gogarten-Boekels M
AD  - Botanisches Institut I der Justus-Liebig-Universitat Senckenbergstrasse 17-21, 
      D-6300 Giessen, F.R.G.
FAU - Gogarten, J P
AU  - Gogarten JP
FAU - Bentrup, F W
AU  - Bentrup FW
LA  - eng
PT  - Journal Article
DEP - 20120120
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 2012/12/01 06:00
PHST- 1984/09/17 00:00 [received]
PHST- 1984/10/31 00:00 [accepted]
PHST- 2012/12/01 06:00 [entrez]
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
AID - S0176-1617(85)80190-5 [pii]
AID - 10.1016/S0176-1617(85)80190-5 [doi]
PST - ppublish
SO  - J Plant Physiol. 1985 Mar;118(4):309-25. doi: 10.1016/S0176-1617(85)80190-5. Epub 
      2012 Jan 20.