PMID- 2319630
OWN - NLM
STAT- MEDLINE
DCOM- 19900507
LR  - 20071115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 25
IP  - 2
DP  - 1990 Feb
TI  - Coexpression of lactosyl and gangliotetraosyl gangliosides in rat cerebellar 
      radial glial cells in culture.
PG  - 214-22
AB  - The expression of gangliosides of the lactosylceramide (LC) and of the 
      gangliotetraosylceramide (GTC) series on the surface of cells from rat embryonic 
      cerebellar tissue was investigated by double-color indirect immunofluorescence. 
      GD3 was assumed to be representative of LC and was detected using a specific 
      monoclonal antibody. GM1 was assumed to be representative of GTC and was detected 
      using the binding of cholera toxin followed by the binding of cholera toxin 
      antibodies. The expression of polysialosylated GTC (polysialosyl-GTC) was 
      detected using the cholera toxin-cholera toxin antibody experimental approach 
      after conversion of polysialosyl-GTC to GM1 by treatment of the cells with 
      neuraminidase. To distinguish the major neural cell types present in the cultures 
      the expression of the following cell type-specific markers was investigated: 
      neuron-specific enolase and microtubule-associated protein-2 (MAP-2) as probes 
      for neuronal cells and the intermediate filament protein glial fibrillar acidic 
      protein (GFAP) as a probe for astroglial cells. More than 80% of cells 
      dissociated from cerebellar tissue of 15-day-old rat embryos (E15) are positive 
      for the expression of GD3 and about 50% for the expression of GM1 and 
      polysialosyl-GTC, but most are negative for the expression of neuron-specific 
      enolase, MAP-2, and GFAP. After culturing for 4 days (E15 + 4) most cells that 
      show characteristics of neuronal cells are positive for the expression of 
      polysialosyl-GTC and "inactivate" the expression of GD3. Most cells with 
      characteristics of radial and stellate glial cells are also positive for the 
      expression of polysialosyl-GTC, but unlike neuron-like cells, they do not 
      "inactivate" the expression of GD3.
FAU - Gravotta, D
AU  - Gravotta D
AD  - Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Argentina.
FAU - Ferreira, A
AU  - Ferreira A
FAU - Busciglio, J
AU  - Busciglio J
FAU - Caceres, A
AU  - Caceres A
FAU - Landa, C A
AU  - Landa CA
FAU - Maccioni, H J
AU  - Maccioni HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Gangliosides)
RN  - 37758-47-7 (G(M1) Ganglioside)
RN  - 62010-37-1 (ganglioside, GD3)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cerebellum/cytology/*metabolism
MH  - Embryo, Mammalian
MH  - G(M1) Ganglioside/*metabolism
MH  - Gangliosides/*metabolism
MH  - Immunohistochemistry
MH  - Neuroglia/cytology/*metabolism
MH  - Rats
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 10.1002/jnr.490250209 [doi]
PST - ppublish
SO  - J Neurosci Res. 1990 Feb;25(2):214-22. doi: 10.1002/jnr.490250209.