PMID- 23197422
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20220408
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 101
IP  - 6
DP  - 2013 Jun
TI  - Collagen-nanofiber hydrogel composites promote contact guidance of human 
      lymphatic microvascular endothelial cells and directed capillary tube formation.
PG  - 1787-99
LID - 10.1002/jbm.a.34468 [doi]
AB  - Collagen and fibronectin matrices are known to stimulate migration of 
      microvascular endothelial cells and the process of tubulogenesis, but the 
      physical, chemical, and topographical cues for directed vessel formation have yet 
      to be determined. In this study, growth, migration, elongation, and tube 
      formation of human lymphatic microvascular endothelial cells (LECs) were 
      investigated on electrospun poly(D,L-lactic-co-glycolic acid) (PLGA) and 
      poly(L-lactic-co-D-lactic acid) (PLDL) nanofiber-coated substrates, and 
      correlated with fiber density and diameter. Directed migration of LECs was 
      observed in the presence of aligned nanofibers, whereas random fiber alignment 
      slowed down migration and growth of LECs. Cell guidance was significantly 
      enhanced in the presence of more hydrophobic PLDL polymer nanofibers compared to 
      PLGA (10:90). Subsequent experiments with tube-forming assays reveal the ability 
      of resorbable hydrophobic nanofibers >300 nm in diameter to promote cell guidance 
      in collagen gels without direct cell-fiber contact, in contrast to the previously 
      reported contact-guidance phenomena. Our results show that endothelial cell 
      guidance is possible within nanofiber/collagen-gel constructs that mimic the 
      native extracellular matrix in terms of size and orientation of fibrillar 
      components.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Laco, Filip
AU  - Laco F
AD  - Department of Biomedical Engineering, University of Strathclyde, Glasgow, 
      Scotland, United Kingdom. filiplaco@gmail.com
FAU - Grant, M Helen
AU  - Grant MH
FAU - Black, Richard A
AU  - Black RA
LA  - eng
PT  - Journal Article
DEP - 20121130
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Actins)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Actins/metabolism
MH  - Capillaries/drug effects/*growth & development
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Collagen/*pharmacology
MH  - Endothelial Cells/*cytology/drug effects/metabolism
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/*pharmacology
MH  - Lactic Acid/pharmacology
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Neovascularization, Physiologic/*drug effects
MH  - Polyglycolic Acid/pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Staining and Labeling
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
EDAT- 2012/12/01 06:00
MHDA- 2013/10/23 06:00
CRDT- 2012/12/01 06:00
PHST- 2012/05/23 00:00 [received]
PHST- 2012/09/06 00:00 [revised]
PHST- 2012/09/21 00:00 [accepted]
PHST- 2012/12/01 06:00 [entrez]
PHST- 2012/12/01 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - 10.1002/jbm.a.34468 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2013 Jun;101(6):1787-99. doi: 10.1002/jbm.a.34468. Epub 
      2012 Nov 30.