PMID- 23198004
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121203
LR  - 20211021
IS  - 1874-2106 (Electronic)
IS  - 1874-2106 (Linking)
VI  - 6
DP  - 2012
TI  - Effect of nickel chloride on cell proliferation.
PG  - 177-81
LID - 10.2174/1874210601206010177 [doi]
AB  - OBJECTIVE: Metal alloys used in dentistry and in other biomedical fields may 
      release nickel ions in the oral environment. The release of nickel might 
      influence the normal biological and physiological processes, including tissue 
      wound healing, cell growth and proliferation. The aim of this study was to 
      evaluate in vitro the effects of nickel ions on cell cycle, viability and 
      proliferation. MATERIALS AND METHODS: Human osteosarcoma cells (U2OS) and human 
      keratinocytes (HaCat) were exposed to different nickel chloride (NiCl(2)) 
      concentrations (0 - 5mM) for various periods exposure. The viability of cultured 
      cells was estimated by flow cytometry using Annexin V-FITC and Propidium Iodide 
      (PI). Cell proliferation was evaluated by using carboxyfluorescein diacetate 
      succinimidyl ester (CFDA-SE) and flow cytometry. Finally, the effects of NiCl(2) 
      on cell cycle were assessed and quantified by flow cytometry. Statistical 
      analysis was performed by means of ANOVA followed by Tukey's test. RESULTS: 
      NiCl(2) induced a dose and time dependent decrease in cell viability. After 24h, 
      1mM NiCl(2) caused a similar and significant reduction of viability in U2OS and 
      HaCat cells, while higher NiCl(2) concentrations and longer exposure times showed 
      a reduced cytotoxic effect in HaCat as compared to U2OS cells. Exposure to 
      NiCl(2) caused a dose- and time-dependent inhibition of cell proliferation in 
      both cell lines tested, with a prominent effect on U2OS cells. Furthermore, both 
      cell lines exposed to NiCl(2) exhibited significant changes in cell cycle 
      distribution after 24h exposure 2mM NiCl2, as compared to untreated cells 
      (p<0.05). CONCLUSION: Our results indicate that release of nickel ions may affect 
      cell proliferation. The inhibition of cell growth by NiCl2 is mediated by both 
      cell cycle arrest and by induction of cell death.
FAU - D'Anto, Vincenzo
AU  - D'Anto V
AD  - Department of Oral and Maxillofacial Sciences, University of Naples "Federico 
      II", Napoli, Italy.
FAU - Valletta, Rosa
AU  - Valletta R
FAU - Amato, Massimo
AU  - Amato M
FAU - Schweikl, Helmut
AU  - Schweikl H
FAU - Simeone, Michele
AU  - Simeone M
FAU - Paduano, Sergio
AU  - Paduano S
FAU - Rengo, Sandro
AU  - Rengo S
FAU - Spagnuolo, Gianrico
AU  - Spagnuolo G
LA  - eng
PT  - Journal Article
DEP - 20121116
PL  - United Arab Emirates
TA  - Open Dent J
JT  - The open dentistry journal
JID - 101480503
PMC - PMC3504722
OTO - NOTNLM
OT  - Biocompatibility
OT  - HaCat.
OT  - NiCl2
OT  - Nickel
OT  - Orthodontic appliances
OT  - U2OS
EDAT- 2012/12/01 06:00
MHDA- 2012/12/01 06:01
PMCR- 2012/01/01
CRDT- 2012/12/01 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2012/10/04 00:00 [revised]
PHST- 2012/10/05 00:00 [accepted]
PHST- 2012/12/01 06:00 [entrez]
PHST- 2012/12/01 06:00 [pubmed]
PHST- 2012/12/01 06:01 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - TODENTJ-6-177 [pii]
AID - 10.2174/1874210601206010177 [doi]
PST - ppublish
SO  - Open Dent J. 2012;6:177-81. doi: 10.2174/1874210601206010177. Epub 2012 Nov 16.