PMID- 23199104
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130108
LR  - 20211021
IS  - 1756-6614 (Electronic)
IS  - 1756-6614 (Linking)
VI  - 5
IP  - 1
DP  - 2012 Nov 30
TI  - Distribution of subpopulations of dendritic cells in peripheral blood of patients 
      treated with exogenous thyrotropin.
PG  - 18
LID - 10.1186/1756-6614-5-18 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) play a major role as regulators of inflammatory 
      events associated with thyroid pathology. The immunoregulatory function of DCs 
      depends strongly on their subtype, as well as maturation and activation status. 
      Numerous hormonal factors modulate the immune properties of DCs, however, little 
      is known about effects exerted by the hypothalamus-pituitary-thyroid-axis. 
      Recently, we have shown a direct regulatory influence of thyroid hormones (TH) on 
      human DCs function. The aim of the present study was to analyze the effect of 
      systemically administered thyrotropin (TSH) on human blood DCs ex vivo. METHODS: 
      Blood samples for the cytometric analysis of peripheral blood plasmacytoid and 
      myeloid DCs subtypes were collected from patients subjected to total 
      thyroidectomy because of differentiated thyroid carcinoma at 2 time points: (i) 
      directly before the commencement of TSH administration and (ii) 5 days after 
      first TSH injection. The whole blood quantitative and phenotypic analysis of 
      plasmacytoid and myeloid DCs subtypes was performed by flow cytometry. RESULTS: 
      Administration of TSH did not influence the percentage of plasmacytoid DCs in 
      peripheral blood of study participants. Also the percentage of the two main 
      myeloid DCs subpopulations - CD1c/BDCA1+ DCs and CD141/BDCA3+ DCs did not change 
      significantly. TSH administration had no effect on the surface expression of CD86 
      - one of the major costimulatory molecules - neither in the whole peripheral 
      blood mononuclear cell (PBMC) fraction nor in particular DCs subtypes. 
      CONCLUSIONS: In the present study, we demonstrated no influence of systemic TSH 
      administration on human peripheral blood DCs subtypes. These results are in 
      accordance with our previous work suggesting the direct effect of TH on human DCs 
      ex vivo.
FAU - Stasiolek, Mariusz
AU  - Stasiolek M
AD  - Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial 
      Hospital - Research Institute, Rzgowska Str, 281/289, 93-338, Lodz, Poland. 
      mstasiolek@yahoo.de.
FAU - Adamczewski, Zbigniew
AU  - Adamczewski Z
FAU - Pula, Bartosz
AU  - Pula B
FAU - Krawczyk-Rusiecka, Kinga
AU  - Krawczyk-Rusiecka K
FAU - Zygmunt, Arkadiusz
AU  - Zygmunt A
FAU - Borowiecka, Magdalena
AU  - Borowiecka M
FAU - Dziegiel, Piotr
AU  - Dziegiel P
FAU - Lewinski, Andrzej
AU  - Lewinski A
LA  - eng
PT  - Journal Article
DEP - 20121130
PL  - England
TA  - Thyroid Res
JT  - Thyroid research
JID - 101469037
PMC - PMC3537577
EDAT- 2012/12/04 06:00
MHDA- 2012/12/04 06:01
PMCR- 2012/11/30
CRDT- 2012/12/04 06:00
PHST- 2012/10/12 00:00 [received]
PHST- 2012/11/14 00:00 [accepted]
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2012/12/04 06:01 [medline]
PHST- 2012/11/30 00:00 [pmc-release]
AID - 1756-6614-5-18 [pii]
AID - 10.1186/1756-6614-5-18 [doi]
PST - epublish
SO  - Thyroid Res. 2012 Nov 30;5(1):18. doi: 10.1186/1756-6614-5-18.