PMID- 23199572
OWN - NLM
STAT- MEDLINE
DCOM- 20130423
LR  - 20161020
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 55
IP  - 11
DP  - 2012 Nov
TI  - EST sequencing and fosmid library construction in a non-model moth, Mamestra 
      brassicae, for comparative mapping.
PG  - 775-81
LID - 10.1139/g2012-063 [doi]
AB  - Genome data are useful for both basic and applied research; however, it is 
      difficult to carry out large-scale genome analyses using species with limited 
      genetic or genomic resources. Here, we describe a cost-effective method to 
      analyze the genome of a non-model species, using the cabbage moth, Mamestra 
      brassicae (Lepidoptera: Noctuidae). First, we conducted expression sequence tag 
      (EST) analysis. In this analysis, we performed PCR-based prescreening of a 
      non-normalized embryonic cDNA library to eliminate already sequenced cDNAs from 
      further sequencing, which significantly increased the percentage of unique genes. 
      Next, we constructed a fosmid library of M. brassicae and isolated 120 clones 
      containing 119 putative single copy genes by PCR-based screening with primer sets 
      designed from the ESTs. Finally, we showed that the isolated fosmid clones could 
      be used as probes for multicolor fluorescence in situ hybridization (FISH) 
      analysis against an M. brassicae chromosome and confirmed conserved gene order 
      between M. brassicae and the silkworm, Bombyx mori. Thus, we developed new 
      genomic resources for comparative genome analysis in M. brassicae using robust 
      and relatively low cost methods that can be applied to any non-model organism.
FAU - Kamimura, Manabu
AU  - Kamimura M
AD  - National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
FAU - Tateishi, Ken
AU  - Tateishi K
FAU - Tanaka-Okuyama, Makiko
AU  - Tanaka-Okuyama M
FAU - Okabe, Takuya
AU  - Okabe T
FAU - Shibata, Fukashi
AU  - Shibata F
FAU - Sahara, Ken
AU  - Sahara K
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121029
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Bombyx/*genetics
MH  - Chromosomes/genetics
MH  - DNA, Complementary/genetics
MH  - Expressed Sequence Tags
MH  - Gene Library
MH  - Genomics/economics/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Moths/*genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Analysis, DNA/economics/*methods
EDAT- 2012/12/04 06:00
MHDA- 2013/04/24 06:00
CRDT- 2012/12/04 06:00
PHST- 2012/12/04 06:00 [entrez]
PHST- 2012/12/04 06:00 [pubmed]
PHST- 2013/04/24 06:00 [medline]
AID - 10.1139/g2012-063 [doi]
PST - ppublish
SO  - Genome. 2012 Nov;55(11):775-81. doi: 10.1139/g2012-063. Epub 2012 Oct 29.